研究等業績 - その他 - 植木　重治

Lytic eosinophils produce extracellular DNA traps as well as free eosinophil granules.

Persson C, Ueki S

The Journal of allergy and clinical immunology ( Elsevier {BV} )  141 ( 3 ) 1164 - 1164   2018年03月

DOI PubMed

Aspergillus Fumigatusによる好酸球ETosisの誘導機構

植木 重治, Neves Josiane, 竹田 正秀, 福地 峰世, 今野 泰典, 蛇澤 晶, 浅野 浩一郎, 廣川 誠

日本呼吸器学会誌 ( (一社)日本呼吸器学会 )  7 ( 増刊 ) 304 - 304   2018年03月

Aspergillus Fumigatusによる好酸球ETosisの誘導機構

植木 重治, Neves Josiane, 竹田 正秀, 福地 峰世, 今野 泰典, 蛇澤 晶, 浅野 浩一郎, 廣川 誠

日本呼吸器学会誌 ( (一社)日本呼吸器学会 )  7 ( 増刊 ) 304 - 304   2018年03月

アレルギー性気管支肺アスペルギルス症の粘液栓における好酸球Extracellular traps

竹田 正秀, 植木 重治, 福地 峰世, 今野 泰典, 面川 歩, 嵯峨 知生, 嵯峨 亜希子, 守時 由起, 廣川 誠, 蛇澤 晶, 浅野 浩一郎

日本呼吸器学会誌 ( (一社)日本呼吸器学会 )  7 ( 増刊 ) 305 - 305   2018年03月

アレルギー性気管支肺アスペルギルス症の粘液栓における好酸球Extracellular traps

竹田 正秀, 植木 重治, 福地 峰世, 今野 泰典, 面川 歩, 嵯峨 知生, 嵯峨 亜希子, 守時 由起, 廣川 誠, 蛇澤 晶, 浅野 浩一郎

日本呼吸器学会誌 ( (一社)日本呼吸器学会 )  7 ( 増刊 ) 305 - 305   2018年03月

Eosinophils release extracellular DNA traps in response to Aspergillus fumigatus

Muniz V.S.

Journal of Allergy and Clinical Immunology ( Journal of Allergy and Clinical Immunology )  141 ( 2 ) 571 - 585.e7   2018年02月

BACKGROUND: Eosinophils mediate the immune response in different infectious conditions. The release of extracellular DNA traps (ETs) by leukocytes has been described as an innate immune response mechanism that is relevant in many disorders including fungal diseases. Different stimuli induce the release of human eosinophil ETs (EETs). Aspergillus fumigatus is an opportunistic fungus that may cause eosinophilic allergic bronchopulmonary aspergillosis (ABPA). It has been reported that eosinophils are important to the clearance of A fumigatus in infected mice lungs. However, the immunological mechanisms that underlie the molecular interactions between A fumigatus and eosinophils are poorly understood. OBJECTIVE: Here, we investigated the presence of EETs in the bronchial mucus plugs of patients with ABPA. We also determined whether A fumigatus induced the release of human eosinophil EETs in vitro. METHODS: Mucus samples of patients with ABPA were analyzed by light and confocal fluorescence microscopy. The release of EETs by human blood eosinophils was evaluated using different pharmacological tools and neutralizing antibodies by fluorescence microscopy and a fluorimetric method. RESULTS: We identified abundant nuclear histone-bearing EETs in the bronchial secretions obtained from patients with ABPA. In vitro, we demonstrated that A fumigatus induces the release of EETs through a mechanism independent of reactive oxygen species but associated with eosinophil death, histone citrullination, CD11b, and the Syk tyrosine kinase pathway. EETs lack the killing or fungistatic activities against A fumigatus. CONCLUSIONS: Our findings may contribute to the understanding of how eosinophils recognize and act as immune cells in response to A fumigatus, which may lead to novel insights regarding the treatment of patients with ABPA.

DOI PubMed

ETosis-derived DNA trap production in middle ear effusion is a common feature of eosinophilic otitis media

Ohta Nobuo, Ueki Shigeharu, Konno Yasunori, Hirokawa Makoto, Kubota Toshinori, Tomioka-Matsutani Sachiko, Suzuki Takahiro, Ishida Yusuke, Kawano Tasuku, Miyasaka Tomomitsu, Takahashi Tomoko, Suzuki Tatsutoshi, Ohno Isao, Kakehata Seiji, Fujieda Shigeharu

Allergology International ( 一般社団法人日本アレルギー学会 )  67 ( 3 ) 414 - 416   2018年

DOI CiNii Research

Mucus plugging in allergic bronchopulmonary aspergillosis: Implication of the eosinophil DNA traps

Omokawa Ayumi, Ueki Shigeharu, Kikuchi Yuta, Takeda Masahide, Asano Mariko, Sato Kazuhiro, Sano Masaaki, Ito Hiroshi, Hirokawa Makoto

Allergology International ( 一般社団法人日本アレルギー学会 )  67 ( 2 ) 280 - 282   2018年

DOI CiNii Research

Evaluating effectiveness of infection control efforts in hospitals using information in microbiological laboratory databases

Saito N.

Journal of General and Family Medicine ( Journal of General and Family Medicine )  18 ( 6 ) 354 - 359   2017年12月

Purpose: To analyze the quality of infection control activities, bacteriological data relevant to infection control was evaluated through the microbiological data warehouse networking hospitals in two medical regions. Methods: Data regarding bacterial test results of 19 hospitals were extracted from two microbiological laboratory information data bases. The rate of MRSA among total S. aureus was used as a general indicator of infection control activities. The occupancy rate of nasal or pharyngeal swabs among MRSA-positive bacteriological samples was used as an indicator of attention paid for infection control in intensive care wards. The number of blood culture sets per examined patient was utilized as an indicator for life-long vocational education on updated medical practice relevant to infectious diseases. Results: The rate of MRSA was significantly higher in secondary private hospitals. The occupancy rate of nasal or pharyngeal swabs was significantly higher in tertiary hospitals. The average number of blood culture set per examined patient were 1.55, 1.54 and 1.39 in tertiary, secondary public and secondary private hospitals, respectively; however, there were no statistical differences between groups. Conclusions: Data bases of microbiological test results shared by hospital laboratories are useful for evaluating regional infection control activities.

DOI PubMed

High-Throughput Sequencing of IgG B-Cell Receptors Reveals the Frequent Usage of the Rearranged IGHV4-28//IGHJ4 Gene in Primary Immune Thrombocytopenia in Adults

Naohito Fujishima, Masaru Togashi, Ryo Hasegawa, Ken Watanabe, Akiko Saga, Ayumi Omokawa, Tomoo Saga, Shigeharu Ueki, Naoto Takahashi, Kazutaka Kitaura, Ryuji Suzuki, Makoto Hirokawa

BLOOD ( AMER SOC HEMATOLOGY )  130   2017年12月

研究発表要旨（国際会議）  

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非医療系学部学生の開発途上国での海外実習に対応した渡航前教育の試み

嵯峨 知生, 面川 歩, 植木 重治, 須田 智美, 遠田 耕平, 長谷川 仁志, 廣川 誠

グローバルヘルス合同大会プログラム・抄録集 ( グローバルヘルス合同大会事務局 )  2017   340 - 340   2017年11月

LETTER TO THE EDITOR 第10回国際好酸球学会シンポジウム

植木 重治, 山田 佳之

臨床免疫・アレルギー科 = Clinical immunology & allergology ( 科学評論社 )  68 ( 4 ) 452 - 454   2017年10月

活性化したヒト好酸球から産生される細胞外小胞の特徴

植木 重治, 竹田 正秀, 面川 歩, 嵯峨 知生, 廣川 誠, Akuthota Praveen, Melo Rossana C.N.

臨床病理 ( 日本臨床検査医学会 )  65 ( 補冊 ) 140 - 140   2017年10月

活性化したヒト好酸球から産生される細胞外小胞の特徴

植木 重治, 竹田 正秀, 面川 歩, 嵯峨 知生, 廣川 誠, Akuthota Praveen, Melo Rossana C.N

臨床病理 ( 日本臨床検査医学会 )  65 ( 補冊 ) 140 - 140   2017年10月

アレルギー・好酸球性炎症の病態解明

植木 重治

秋田医学 ( 秋田医学会 )  44 ( 1 ) 11 - 17   2017年06月

国民の半数近くが何らかのアレルギー性疾患を有するとされ,その病態を明らかにすることは社会的な要請である.アレルギーを理解する上で,慢性炎症の概念は欠くことができない.多くの病態で好酸球を中心とした炎症細胞が組織に集積し,障害をきたすことが問題となっている.好酸球は一方で,寄生虫や病原微生物に対する免疫,組織改変や恒常性の維持など多才な役割を担う細胞であり,好酸球の状態を適切に把握し治療に応用することが求められている.(著者抄録)

造血不全における平均血小板容積(MPV)および幼若血小板比率(IPF)の臨床的有用性とカットオフ値に関する検討

菊地 優子, 永沼 綾子, 長谷山 佳菜, 小野 杏子, 小林 毅, 小林 則子, 面川 歩, 嵯峨 知生, 植木 重治, 廣川 誠

日本検査血液学会雑誌 ( (一社)日本検査血液学会 )  18 ( 学術集会 ) S215 - S215   2017年06月

Eosinophilic Otitis Media: the Aftermath of Eosinophil Extracellular Trap Cell Death

Ueki S.

Current Allergy and Asthma Reports ( Current Allergy and Asthma Reports )  17 ( 5 ) 33   2017年05月

Purpose of Review Eosinophilic otitis media (EOM) is a refractory disease characterized by the accumulation of eosinophils in middle ear effusion and mucosa. We summarize current knowledge regarding the clinical characteristics and management of EOM. Although eosinophil activation in inflamed foci is involved in the pathogenesis of EOM, little is known about the fate of the eosinophils and aftermath of their cell death. We discuss the possibility that eosinophils undergo non-apoptotic cell death that worsens tissue damage and increases effusion viscosity.
Recent Findings Unlike chronic otitis media, EOM is strongly associated with an allergic background. Corticosteroids are currently the only effective pharmacological treatment, and surgical intervention is often required. Mucosal eosinophils infiltrate extensively into the middle ear cavity where they are stimulated by locally produced activators including interleukin-5 and eotaxin. The eosinophils undergo cytolysis in the effusion, which represents a major fate of activated eosinophils in vivo. Recent data revealed cytolysis could be renamed as extracellular trap cell death (ETosis). ETosis represents suicidal cell death involving total cell degranulation and development of sticky chromatin structures (extracellular traps (ETs)). The characteristics of eosinophil-and neutrophil-derived ET polymers might contribute to the difference in viscosity of secretions between EOM and common chronic otitis media.
Summary The extracellular products remaining after eosinophil ETosis are an important aspect of EOM pathology. The concept of ETosis also has novel implications for potential therapeutic modalities in various eosinophilic disorders.

DOI PubMed

喘息合併好酸球性副鼻腔炎の加療中に発症した好酸球性胆嚢炎の一症例

桑原 敏彰, 小林 良樹, 尹 泰貴, 神田 晃, 岩井 大, 廣川 誠, 朝子 幹也, 植木 重治

アレルギー ( (一社)日本アレルギー学会 )  66 ( 4-5 ) 704 - 704   2017年05月

好酸球、T細胞、抗原提示細胞 ヒト好酸球から産生されるextracellular microvesicles

植木 重治, 竹田 正秀, 中村 由夏, 丹 典子, 廣川 誠, Akuthota Praveen, Weller Peter, Melo Rosanna

アレルギー ( (一社)日本アレルギー学会 )  66 ( 4-5 ) 594 - 594   2017年05月

好酸球の新しい細胞死 : ETosis (特集 好酸球性炎症 アップデート)

竹田 正秀, 植木 重治, 廣川 誠

アレルギー・免疫 ( 医薬ジャーナル社 )  24 ( 5 ) 612 - 617   2017年05月