研究者詳細 - 南條　博

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ニボルマブが奏功した転移性 Mucinous tubular and spindle cell carcinoma の1例

髙橋佳子, 沼倉一幸, 青山有, 岡田脩平, 齋藤拓郎, 武藤弓奈, 小泉淳, 奈良健平, 千葉修治, 神田壮平, 齋藤満, 成田伸太郎, 井上高光, 佐藤滋, 南條博, 羽渕友則

泌尿器科紀要 ( 泌尿器科紀要刊行会 ) 65 ( 9 ) 363 - 367 2019年09月

DOI CiNii Research

電界撹拌技術を用いた酵素免疫測定法の迅速化技術の開発（第四報）

中村竜太, 大久保義真, 久住孝幸, 南條博, 南谷佳弘, 赤上陽一

精密工学会学術講演会講演論文集 ( 公益社団法人精密工学会 ) 2019S ( 0 ) 327 - 328 2019年03月

我々は電界砥粒制御技術を応用し，微小量の液滴を非接触に撹拌可能な技術を開発，酵素免疫測定法（ELISA）へ電界撹拌技術を導入し，迅速なELISA技術を提案している．本研究ではマイクロウェルプレート間における電界撹拌のばらつきを少なくし，撹拌の均一化を図るため，最適電界印加方法について検討した．結果として，一体型電極から分割型電極にすることによりプレート間の電界撹拌のばらつきを抑えることに成功した．

DOI CiNii Research

Radical Prostatectomy With and Without Neoadjuvant Chemohormonal Pretreatment for High-Risk Localized Prostate Cancer: A Comparative Propensity Score Matched Analysis

Narita S.

Clinical Genitourinary Cancer ( Clinical Genitourinary Cancer ) 17 ( 1 ) e113 - e122 2019年02月

DOI

Reagent-saving immunohistochemistry for HER2 using non-contact alternating current electric field mixing

Hoshino I.

Journal of Clinical Pathology ( Journal of Clinical Pathology ) 72 ( 1 ) 25 - 30 2019年01月

DOI

Clinical Significance of Molecular Diagnosis of Pilocytic Astrocytoma: A Case Report

Ono Takahiro, Takahashi Masataka, Hatakeyama Junya, Oda Masaya, Sahm Felix, Nanjo Hiroshi, von Deimling Andreas, Shimizu Hiroaki

NMC Case Report Journal ( 一般社団法人日本脳神経外科学会 ) 6 ( 3 ) 95 - 99 2019年

This paper reports on a case of pilocytic astrocytoma (PA), for which a diagnosis by conventional pathological diagnosis was difficult but an accurate diagnosis was possible by a new molecular diagnostic method. A 13-year-old girl whose tumor developed by a headache that gradually worsened, and a well-demarcated T2-hyperintense lesion was found in the left cerebellum by a magnetic resonance imaging while the apparent diffusion coefficient value was also high. While the finding was a typical PA, histological features of PA were not found in the surgical specimen. An initial diagnosis was anaplastic astrocytoma (AA), and the final diagnosis through a central review was diffuse astrocytoma (DA). On the other hand, using MethylationEPIC (850 K) array, an analysis by a DNA methylation-based tumor classifier tool as reported by Capper et al. showed that this case belonged to a methylation class of PA. The copy number profile calculated from the methylation array data showed hints of BRAF/KIAA1549 fusion and no other chromosomal alterations, which also supported the molecular diagnosis. The patient was treated with local radiotherapy concomitant with temozolomide based on the initial pathological diagnosis during the consultation, but maintenance temozolomide therapy was not done according to the final molecular diagnosis. The tumor showed no recurrence for 20 months. In this case, the integrated diagnostic approach based on histological and molecular findings was clinically significant to select proper adjuvant treatment. It is crucial that the usefulness and robustness of this new molecular diagnostic method be validated further.

DOI

手術中に病気を視る迅速免疫染色入門

南條博, 赤上陽一, 中村竜太, 廣嶋優子, 寺田かおり, 今井一博, 南谷佳弘

年次大会 ( 一般社団法人日本機械学会 ) 2019 ( 0 ) 2019年

Immunohistochemistry (IHC) is the most common application of immunostaining. It is widely used in the disgnosis of abnormal cells such as those found in cancerous tumors. Intraoperative pathological diagnosis is also important in selecting suitable surgery for cancer patients. We performed intraoperative diagnosis of 830 cases in Akita University Hospital., with a novel method of rapid-immunohistochemistory (R-IHC) using an alternating current electric field. The results were very good, R-IHC detected an objective evidence that were undetected using conventional HE staining. So we can diagnose degree of malignancy, histological type of cancer, and lymph node metastasis more accurately.

DOI

病理診断用プレパラート作成のための薄切片の切削機構の検討

中西将也, 吉野雅彦, 中川佑貴, 南條博, 赤上陽一, 中村竜太

生産加工・工作機械部門講演会 : 生産と加工に関する学術講演会 ( 一般社団法人日本機械学会 ) 2019 ( 0 ) 2019年

In pathological diagnosis, it is necessary that the tumors are picked out by an operation, embedded in paraffin and sliced in thickness of around 2~5µm to prepare the slide glass. This slicing process is one of the important technologies that affect the accuracy of diagnosis. In this report, the mechanism of a micro slicing process of paraffin blocks was studied. As the temperature of paraffin blocks decrease, cutting force increases. Also, it doesn’t become zero where the setting depth of cut is zero due to tool edge roundness, and the model considering it was made.

DOI

術中迅速免疫組織染色を実現させる電界撹拌技術の開発

中村竜太, 大久保義真, 南條博, 南谷佳弘, 久住貴幸, 赤上陽一

年次大会 ( 一般社団法人日本機械学会 ) 2019 ( 0 ) 2019年

We aim to develop innovative stirring technology (electric field mixing (EFM)) applied abrasive control technique with AC electric field. This technique allows for stirring without contact by applying AC electric field to droplets. This technique was applied to intraoperative immunohistochemical staining. Under EFM showed antigen-antibody reaction time in immunohistochemical staining can be shortened from 90 minutes of conventional method to 10 minutes. In this study, EFM examined the mechanism which makes antigen-antibody reaction of an immunohistochemical staining quicken. As a result, we obtained that it has the mechanism which a reaction time of an immunohistochemical staining is shorten because antigens and antibodies improve to increase the frequencies of contact, and further antibodies disperse and becomes easy to react to antigens under EFM. compared with conventional method.

DOI

電界撹拌技術をEnzyme-Linked ImmunoSorbent Assay法を用いた臨床検査へ応用するための装置および試薬キットの開発

林秀洋, 中村竜太, 星野育, 南條博, 南谷佳弘, 赤上陽一

年次大会 ( 一般社団法人日本機械学会 ) 2019 ( 0 ) 2019年

DOI

電界撹拌技術を用いた抗原抗体反応の迅速メカニズムの解明

中村竜太, 久住孝幸, 大久保義真, 南條博, 南谷佳弘, 赤上陽一

精密工学会誌 ( 公益社団法人精密工学会 ) 85 ( 2 ) 208 - 212 2019年

By applied electric field mixing (EFM) showed antigen-antibody reaction time in an immunohistochemical staining can be shortened from 90 minutes of conventional method to 10 minutes. In this study, EFM examined the mechanism which makes antigen-antibody reaction of an immunohistochemical staining quicken. As a result, we obtained that it has the mechanism which a reaction time of an immunohistochemical staining is shorten because antigen and antibody improve to increase the frequencies of contact under EFM compared with conventional method. Further, we investigated the effect of AC electric field is applied on antibody. As a result, it found that by applied AC electric field to antibody liquid, antibody dispersed and stabilized, therefore, staining quality is improved.

DOI

電界撹拌技術を用いた酵素免疫測定法の迅速化技術の開発（第四報）:マイクロウェルプレート間における電界撹拌均一化のための最適電界印加方法の検討

中村竜太, 大久保義真, 久住孝幸, 南條博, 南谷佳弘, 赤上陽一

精密工学会学術講演会講演論文集 ( 公益社団法人精密工学会 ) 2019 ( 0 ) 327 - 328 2019年

DOI

手術中に病気を視る迅速免疫染色入門

南條博, 赤上陽一, 中村竜太, 廣嶋優子, 寺田かおり, 今井一博, 南谷佳弘

年次大会 ( 一般社団法人日本機械学会 ) 2019 ( 0 ) W02101 2019年

DOI CiNii Research

病理診断用プレパラート作成のための薄切片の切削機構の検討

中西将也, 吉野雅彦, 中川佑貴, 南條博, 赤上陽一, 中村竜太

生産加工・工作機械部門講演会 : 生産と加工に関する学術講演会 ( 一般社団法人日本機械学会 ) 2019.13 ( 0 ) A37 2019年