Research Achievements - Other -

Investigation of Paraffin Micro Cutting Mechanism for Production of Pathological Diagnosis

OMURA Toru, YOSHINO Masahiko, KUSUMI Takayuki, NAKAMURA Ryuta, AKAGAMI Yoichi, NANJO Hiroshi

The Proceedings of The Manufacturing & Machine Tool Conference ( The Japan Society of Mechanical Engineers )  2018 ( 0 )   2018

For pathological diagnosis, it is necessary to slice tissues embedded in paraffin and prepare a slide glass of the section. Slicing is a fine cutting process with a depth of cut with a few micrometers, and there are many unclear points about the mechanism and the distortion inside the thin sections. Therefore, this study aims to investigate the thinning mechanism in detail using a paraffin block. In this report, we report the effect of cutting conditions on the cutting data and morphology of thin sections measured using the tissue slicer we have developed.

DOI

Study on determining various conditions for rapid protocol using Electric field Stirring Technique (EST):Effect of applied frequency on droplet behavior

Akagami Koji, Nakamura Ryuta, Nanjo Hiroshi, Minamiya Yoshihiro

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2018 ( 0 ) 481 - 482   2018

DOI

Study on transpiration suppression of reagents for applying electric field stirring technique (EST) to rapid in situ hybridization (part.2):Investigation of transpiration suppression method by physical approach

Nakamura Ryuta, Akagami Koji, Fujishima Satoshi, Kusumi Takayuki, Nanjo Hiroshi, Minamiya Yoshihiro, Akagami Yoichi

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2018 ( 0 ) 483 - 484   2018

DOI

Development of electric field stirring technology (EST) applied abrasive control technique with AC electric field (Part .3):- Investigation of stirring effect using fluorescently labeled antibody protein -

NAKAMURA Ryuta, KUSUMI Takayuki, OKUBO Yoshinobu, NANJO Hiroshi, MINAMIYA Yoshihiro, AKAGAMI Yoichi

The Proceedings of The Manufacturing & Machine Tool Conference ( The Japan Society of Mechanical Engineers )  2018 ( 0 )   2018

We have developed an innovative stirring technology (electric field stirring technology (EST)) that enables stirring without contact by applying electric field abrasive grain control technology. In the previous study, we reported the characteristics of the EST and the shape of the electrode for efficient EST. In this study, the effect of stirring by EST was investigated. Using fluorescently labeled antibody, efficient diffusion and rapid antigen - antibody reaction were examined by microscopic observation and measurement using flow cytometer, respectively. As a result, it was found from the microscope observation that EST increased the antibody moving velocity by about 22 times compared to static condition. Furthermore, from the measurement results using flow cytometer, it was found that the reaction was accelerated 6 to 10 times by using miniaturization and electric field stirring. From these results, we have experimentally clarified the stirring effect.

DOI

Prevention of Evaporation Caused by a Rapid Immunohistochemistry Method Applying Non-contact Alternating-Current Electric-Field Mixing

HOSHINO Iku, SATO Yusuke, MOTOYAMA Satoru, NANJO Hiroshi, AKAGAMI Yoichi, MINAMIYA Yoshihiro, IMAI Kazuhiro, NAKAMURA Ryuta, SAITO Yoshitaro, FUJISHIMA Satoshi, KURIHARA Nobuyasu, Wakamatsu Yuki, SAITO Hajime, TERATA Kaori

Journal of the Japan Society for Precision Engineering ( The Japan Society for Precision Engineering )  84 ( 4 ) 383 - 387   2018

<p>It is widely recognized that pathology is the most important factor for staging and selecting effective chemotherapy for patients with cancer. Immunohistochemistry (IHC) is a reliable screening method, but intra-operative diagnosis by frozen section with IHC is not possible because IHC takes approximately 6 hours. We recently developed a rapid-IHC method that makes use of an alternating current (AC) electric field to facilitate the antigen-antibody reaction, and reported its usefulness for detection of lung cancer and breast cancer. The antibody is mixed within microdroplets as the voltage is switched on and off at specific intervals. The resultant Coulomb force stirs the diluted solution on the sections, which increases the opportunity for contact. The rapid-IHC device reduces the time required for IHC as well as the amount of antibody required for analyses. The device may also be applied for in situ hybridization (ISH); however, ISH requires a warm temperature, such as 37℃, and a longer processing time than the rapid-IHC method. Consequently, evaporation can be a problem during ISH. The aim of the present study was to evaluate the utility and reliability of an oil cover and cover cap made of polyethylene terephthalate (PET) for preventing evaporation due to the AC electric field applied to facilitate the antigen-antibody reaction. We have shown that both the oil cover and PET cover cap prevented evaporation when using the rapid-IHC device.</p>

DOI

Investigation of Paraffin Micro Cutting Mechanism for Production of Pathological Diagnosis

OMURA Toru, YOSHINO Masahiko, KUSUMI Takayuki, NAKAMURA Ryuta, AKAGAMI Yoichi, NANJO Hiroshi

The Proceedings of The Manufacturing & Machine Tool Conference ( The Japan Society of Mechanical Engineers )  2018.12 ( 0 ) A03   2018

For pathological diagnosis, it is necessary to slice tissues embedded in paraffin and prepare a slide glass of the section. Slicing is a fine cutting process with a depth of cut with a few micrometers, and there are many unclear points about the mechanism and the distortion inside the thin sections. Therefore, this study aims to investigate the thinning mechanism in detail using a paraffin block. In this report, we report the effect of cutting conditions on the cutting data and morphology of thin sections measured using the tissue slicer we have developed.

DOI CiNii Research

Development of electric field stirring technology (EST) applied abrasive control technique with AC electric field (Part .3)

NAKAMURA Ryuta, KUSUMI Takayuki, OKUBO Yoshinobu, NANJO Hiroshi, MINAMIYA Yoshihiro, AKAGAMI Yoichi

The Proceedings of The Manufacturing & Machine Tool Conference ( The Japan Society of Mechanical Engineers )  2018.12 ( 0 ) B02   2018

We have developed an innovative stirring technology (electric field stirring technology (EST)) that enables stirring without contact by applying electric field abrasive grain control technology. In the previous study, we reported the characteristics of the EST and the shape of the electrode for efficient EST. In this study, the effect of stirring by EST was investigated. Using fluorescently labeled antibody, efficient diffusion and rapid antigen - antibody reaction were examined by microscopic observation and measurement using flow cytometer, respectively. As a result, it was found from the microscope observation that EST increased the antibody moving velocity by about 22 times compared to static condition. Furthermore, from the measurement results using flow cytometer, it was found that the reaction was accelerated 6 to 10 times by using miniaturization and electric field stirring. From these results, we have experimentally clarified the stirring effect.

DOI CiNii Research

Investigation of the mechanism for rapid antigen-antibody reaction using electric field stirring technology (E.S.T.)(part.6):Qualitative evaluation of antigen-antibody reaction using flow cytometer

Nakamura Ryuta, Kusumi Takayuki, Nanjo Hiroshi, Minamiya Yoshihiro, Akagami Yoichi

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2017 ( 0 ) 903 - 904   2017

DOI

Study on transpiration suppression of reagents for applying electric field stirring technology (EST) to rapid in situ hybridization

Nakamura Ryuta, Saito Yoshitaro, Kusumi Takayuki, Nanjo Hiroshi, Minamiya Yoshihiro, Akagami Yoichi

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2017 ( 0 ) 365 - 366   2017

DOI

Investigation of the mechanism for rapid antigen-antibody reaction using electric field stirring technology (E.S.T.)(part.6)

Nakamura Ryuta, Kusumi Takayuki, Nanjo Hiroshi, Minamiya Yoshihiro, Akagami Yoichi

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2017A ( 0 ) 903 - 904   2017

DOI CiNii Research

Study on transpiration suppression of reagents for applying electric field stirring technology (EST) to rapid in situ hybridization

Nakamura Ryuta, Saito Yoshitaro, Kusumi Takayuki, Nanjo Hiroshi, Minamiya Yoshihiro, Akagami Yoichi

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2017S ( 0 ) 365 - 366   2017

DOI CiNii Research