Research Achievements - Other -

A CASE OF ENDOSCOPICALLY RESECTED GIANT BRUNNER'S GLAND HYPERPLASIA RESULTING IN DUODENAL INCARCERATION (WITH A VIDEO)

SATO Masao, YOSHIDA Tatsuki, MATSUHASHI Tamotsu, TAKAHASHI So, FUKUDA Sho, NANJO Hiroshi, HIROSHIMA Yuko, IIJIMA Katsunori

GASTROENTEROLOGICAL ENDOSCOPY ( Japan Gastroenterological Endoscopy Society )  67 ( 2 ) 140 - 148   2025

<p>Here, we present a case of giant Brunnerʼs gland hyperplasia. Although it is often discovered as a submucosal lesion in the duodenum, a definitive diagnosis is challenging. Moreover, if the lesion is large or tends to increase in size, it may be difficult to distinguish it from a malignant neoplasm or it may result in an impaired passage or a complicated obstruction. A74-year-old woman had a 60-mm-sized submucosal tumor in the duodenal bulb, which was suspected to be Brunnerʼs gland hyperplasia. However, the patient did not wish to undergo treatment at that time, which led to the decision of just observing the patient. Regular follow-ups were performed; however, the lesion did not increase in size or cause any subjective symptoms. Eleven years later, the patient presented with epigastric pain. Contrast-enhanced CT and emergency esophagogastroduodenoscopy revealed the same lesion in the duodenal descending part. The lesion was resected via endoscopic submucosal dissection in the presence of a gastroenterology surgeon. Pathological examination revealed a 75×32×26 mm Brunnerʼs gland hyperplasia lesion. Since giant Brunnerʼs gland hyperplasia may cause obstruction or incarceration in the future, early prophylactic resection could be considered as a treatment option.</p>

DOI CiNii Research

Durability test of microtome blades in sectioning of demineralized porcine rib samples

Sasaki Takehiro, Satoh Hirotaka, Nakamae Keito, Nanjo Hiroshi, Akagami Yoichi, Nakamura Ryuta, Kusumi Takayuki, Yoshino Masahiko

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2024S ( 0 ) 383 - 384   2024.02

<p>This paper reports the results of an investigation into the factors affecting the durability of the microtome blade used for sectioning pathology samples. Experiments were carried out to repeated sectioning of demineralized porcine ribs at a constant cutting thickness using an originally developed sectioning apparatus. Changes in cutting force, damage to the cutting edge and quality of the section due to repeated sectioning were measured, and the effects of microtome blade hardness, cutting edge angle and specimen characteristics on the durability of the microtome blade were investigated.</p>

DOI CiNii Research

Durability test of microtome blades in sectioning of bone tissue embedded samples

Satoh Hirotaka, Nakamae Keito, Sasaki Takehiro, Nanjo Hiroshi, Akagami Yoichi, Nakamura Ryuta, Kusumi Takayuki, Yoshino Masahiko

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2023A ( 0 ) 762 - 763   2023.08

<p>This paper reports the results of an investigation into the durability of microtome blades used for sectioning pathological samples. We conducted repeated sectioning experiments at a constant set thickness with automatic sectioning equipment we developed, using paraffin blocks embedded with porcine rib tissue as samples. We measured changes in cutting force and damage to the blade edge and examined the effects on the tissue quality of sections.</p>

DOI CiNii Research

A Case of Renal Anastomosing Hemangioma

SASAKI Yoshiki, KASHIMA Soki, KOYAMA Takashi, HIROSHIMA Yuko, AMANO Kenji, TAKAHASHI Syuhei, NARA Taketoshi, KOIZUMI Atsushi, YAMAMOTO Ryohei, NUMAKURA Kazuyuki, SAITO Mitsuru, NARITA Shintaro, NANJO Hiroshi, SATOH Shigeru, HABUCHI Tomonori

  68 ( 8 ) 265 - 269   2022.08

DOI CiNii Research

Durability test of microtome blade for tissue sectioning

Satoh Hirotaka, Nakamae Keito, Nakagawa Yuki, Nanjo Hiroshi, Akagami Yoichi, Nakamura Ryuta, Kusumi Takayuki, Yoshino Masahiko

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2022A ( 0 ) 278 - 279   2022.08

DOI CiNii Research

Study of cutting force and section thickness for pathological diagnosis

Satoh Hirotaka, Kitamura Shunsuke, Nakagawa Yuuki, Nanjo Hiroshi, Akagami Yoichi, Nakamura Ryuta, Kuzumi Takayuki, Yoshino Masahiko

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2022S ( 0 ) 503 - 504   2022.03

<p>For pathological diagnosis of cancer and other diseases, it is necessary to embed diseased tissues in paraffin and cut them into sections that have thickness of several micrometers to get preparations for microscopic observation. In this study, we aim to improve and stabilize the quality of pathological sections. In this paper, we will report the results of experimental measurements of cutting force and thickness of pathological sections of various specimen-embedded paraffin blocks and the investigation of factors affecting the cutting force and thickness based on mechanical model.</p>

DOI CiNii Research

METASTATIC Mucinous Tubular and Spindle Cell Carcinoma Treated with Nivolumab Successfully : A Case Report

Takahashi Yoshiko, Numakura Kazuyuki, Aoyama Yu, Okada Shuhei, Saito Takuro, Muto Yumina, Koizumi Atsushi, Nara Taketoshi, Chiba Syuji, Kanda Sohei, Saito Mitsuru, Narita Shintaro, Inoue Takamitsu, Satoh Shigeru, Nanjo Hiroshi, Habuchi Tomonori

  65 ( 9 ) 363 - 367   2019.09

A 68-year-old man was referred to our hospital with a right-sided renal tumor identified by ultrasonography at the time of his medical check-up. Computed tomography revealed a well-circumscribed but distorted mass measuring 64×45×57 mm in the right kidney with para-aortic lymph node swelling. Laparoscopic right nephrectomy with para-aortic lymphadenectomy was performed. Histopathological diagnosis was mucinous tubular and spindle cell carcinoma (MTSCC) (pT3a) without lymph node metastasis (pN0). Postoperatively, metastases were identified in the lungs, and the vertebral and iliac bones. The patient was treated with axitinib. The lung nodule progressed and left sacrum metastases appeared even after treatment with axitinib. Therefore, nivolumab was administered as second-line treatment. The metastases in the lungs, as well as vertebral and iliac bone showed complete response to this therapy. MTSCC of the kidney is a rare low-grade renal cell carcinoma without any established systemic therapy for metastatic or unresectable lesions. We report a case of metastatic MTSCC in a patient who showed a favorable response to nivolumab treatment. This is the first report to describe successful treatment of metastatic MTSCC with anti-programmed cell death 1 antibody.

DOI CiNii Research

Development of rapid technology for enzyme linked immunosorbent assay using electric field mixing (EFM) (Part .4)

Nakamura Ryuta, Okubo Yoshinobu, Kusumi Takayuki, Nanjo Hiroshi, Minamiya Yoshihiro, Akagami Yoichi

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2019S ( 0 ) 327 - 328   2019.03

DOI CiNii Research

Intraoperative diagnosis with novel rapid-immunohistochemistry (R-IHC)

NANJO Hiroshi, AKAGAMI Yoichi, NAKAMURA Rhuta, HIROSHIMA Yuko, TERADA Kaori, IMAI Kazuhiro, Minamiya Yoshihiro

The Proceedings of Mechanical Engineering Congress, Japan ( The Japan Society of Mechanical Engineers )  2019 ( 0 )   2019

<p>Immunohistochemistry (IHC) is the most common application of immunostaining. It is widely used in the disgnosis of abnormal cells such as those found in cancerous tumors. Intraoperative pathological diagnosis is also important in selecting suitable surgery for cancer patients. We performed intraoperative diagnosis of 830 cases in Akita University Hospital., with a novel method of rapid-immunohistochemistory (R-IHC) using an alternating current electric field. The results were very good, R-IHC detected an objective evidence that were undetected using conventional HE staining. So we can diagnose degree of malignancy, histological type of cancer, and lymph node metastasis more accurately.</p>

DOI

Study on Slicing Mechanism of Thin Section for Paraffin Slide Glass Production in Pathological Diagnosis

NAKANISHI Masaya, YOSHINO Masahiko, NAKAGAWA Yuki, NANJO Hiroshi, AKAGAMI Yoichi, NAKAMURA Ryuta

The Proceedings of The Manufacturing & Machine Tool Conference ( The Japan Society of Mechanical Engineers )  2019 ( 0 )   2019

In pathological diagnosis, it is necessary that the tumors are picked out by an operation, embedded in paraffin and sliced in thickness of around 2~5µm to prepare the slide glass. This slicing process is one of the important technologies that affect the accuracy of diagnosis. In this report, the mechanism of a micro slicing process of paraffin blocks was studied. As the temperature of paraffin blocks decrease, cutting force increases. Also, it doesn't become zero where the setting depth of cut is zero due to tool edge roundness, and the model considering it was made.

DOI

Development of electric field mixing (EFM) to realize rapid intraoperative immunohistochemical staining

NAKAMURA Ryuta, OKUBO Yoshinobu, NANJO Hiroshi, MINAMIYA Yoshihiro, KUSUMI Takayuki, AKAGAMI Yoichi

The Proceedings of Mechanical Engineering Congress, Japan ( The Japan Society of Mechanical Engineers )  2019 ( 0 )   2019

<p>We aim to develop innovative stirring technology (electric field mixing (EFM)) applied abrasive control technique with AC electric field. This technique allows for stirring without contact by applying AC electric field to droplets. This technique was applied to intraoperative immunohistochemical staining. Under EFM showed antigen-antibody reaction time in immunohistochemical staining can be shortened from 90 minutes of conventional method to 10 minutes. In this study, EFM examined the mechanism which makes antigen-antibody reaction of an immunohistochemical staining quicken. As a result, we obtained that it has the mechanism which a reaction time of an immunohistochemical staining is shorten because antigens and antibodies improve to increase the frequencies of contact, and further antibodies disperse and becomes easy to react to antigens under EFM. compared with conventional method.</p>

DOI

Development of equipment and reagent kit for applying electric field mixing to clinical test using enzyme-linked immunosorbent assay.

HAYASHI Hidehiro, NAKAMURA Ryuta, HOSHINO Iku, NANJO Hiroshi, MINAMIYA Yoshihiro, AKAGAMI Yoichi

The Proceedings of Mechanical Engineering Congress, Japan ( The Japan Society of Mechanical Engineers )  2019 ( 0 )   2019

DOI

Investigation of the Mechanism for Rapid Antigen-Antibody Reaction Using Electric Field Mixing (EFM)

NAKAMURA Ryuta, KUSUMI Takayuki, OKUBO Yoshinobu, NANJO Hiroshi, MINAMIYA Yoshihiro, AKAGAMI Yoichi

Journal of the Japan Society for Precision Engineering ( The Japan Society for Precision Engineering )  85 ( 2 ) 208 - 212   2019

<p>By applied electric field mixing (EFM) showed antigen-antibody reaction time in an immunohistochemical staining can be shortened from 90 minutes of conventional method to 10 minutes. In this study, EFM examined the mechanism which makes antigen-antibody reaction of an immunohistochemical staining quicken. As a result, we obtained that it has the mechanism which a reaction time of an immunohistochemical staining is shorten because antigen and antibody improve to increase the frequencies of contact under EFM compared with conventional method. Further, we investigated the effect of AC electric field is applied on antibody. As a result, it found that by applied AC electric field to antibody liquid, antibody dispersed and stabilized, therefore, staining quality is improved.</p>

DOI

Development of rapid technology for enzyme linked immunosorbent assay using electric field mixing (EFM) (Part .4):Investigation of optimal method of applied electric field for homogenization of electric field mixing between micro well plates

Nakamura Ryuta, Okubo Yoshinobu, Kusumi Takayuki, Nanjo Hiroshi, Minamiya Yoshihiro, Akagami Yoichi

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2019 ( 0 ) 327 - 328   2019

DOI

Intraoperative diagnosis with novel rapid-immunohistochemistry (R-IHC)

NANJO Hiroshi, AKAGAMI Yoichi, NAKAMURA Rhuta, HIROSHIMA Yuko, TERADA Kaori, IMAI Kazuhiro, Minamiya Yoshihiro

The Proceedings of Mechanical Engineering Congress, Japan ( The Japan Society of Mechanical Engineers )  2019 ( 0 ) W02101   2019

<p>Immunohistochemistry (IHC) is the most common application of immunostaining. It is widely used in the disgnosis of abnormal cells such as those found in cancerous tumors. Intraoperative pathological diagnosis is also important in selecting suitable surgery for cancer patients. We performed intraoperative diagnosis of 830 cases in Akita University Hospital., with a novel method of rapid-immunohistochemistory (R-IHC) using an alternating current electric field. The results were very good, R-IHC detected an objective evidence that were undetected using conventional HE staining. So we can diagnose degree of malignancy, histological type of cancer, and lymph node metastasis more accurately.</p>

DOI CiNii Research

Study on Slicing Mechanism of Thin Section for Paraffin Slide Glass Production in Pathological Diagnosis

NAKANISHI Masaya, YOSHINO Masahiko, NAKAGAWA Yuki, NANJO Hiroshi, AKAGAMI Yoichi, NAKAMURA Ryuta

The Proceedings of The Manufacturing & Machine Tool Conference ( The Japan Society of Mechanical Engineers )  2019.13 ( 0 ) A37   2019

In pathological diagnosis, it is necessary that the tumors are picked out by an operation, embedded in paraffin and sliced in thickness of around 2~5µm to prepare the slide glass. This slicing process is one of the important technologies that affect the accuracy of diagnosis. In this report, the mechanism of a micro slicing process of paraffin blocks was studied. As the temperature of paraffin blocks decrease, cutting force increases. Also, it doesn't become zero where the setting depth of cut is zero due to tool edge roundness, and the model considering it was made.

DOI CiNii Research

Development of electric field mixing (EFM) to realize rapid intraoperative immunohistochemical staining

NAKAMURA Ryuta, OKUBO Yoshinobu, NANJO Hiroshi, MINAMIYA Yoshihiro, KUSUMI Takayuki, AKAGAMI Yoichi

The Proceedings of Mechanical Engineering Congress, Japan ( The Japan Society of Mechanical Engineers )  2019 ( 0 ) S00108   2019

<p>We aim to develop innovative stirring technology (electric field mixing (EFM)) applied abrasive control technique with AC electric field. This technique allows for stirring without contact by applying AC electric field to droplets. This technique was applied to intraoperative immunohistochemical staining. Under EFM showed antigen-antibody reaction time in immunohistochemical staining can be shortened from 90 minutes of conventional method to 10 minutes. In this study, EFM examined the mechanism which makes antigen-antibody reaction of an immunohistochemical staining quicken. As a result, we obtained that it has the mechanism which a reaction time of an immunohistochemical staining is shorten because antigens and antibodies improve to increase the frequencies of contact, and further antibodies disperse and becomes easy to react to antigens under EFM. compared with conventional method.</p>

DOI CiNii Research

Development of equipment and reagent kit for applying electric field mixing to clinical test using enzyme-linked immunosorbent assay.

HAYASHI Hidehiro, NAKAMURA Ryuta, HOSHINO Iku, NANJO Hiroshi, MINAMIYA Yoshihiro, AKAGAMI Yoichi

The Proceedings of Mechanical Engineering Congress, Japan ( The Japan Society of Mechanical Engineers )  2019 ( 0 ) S00109   2019

DOI CiNii Research

Study on determining various conditions for rapid protocol using Electric field Stirring Technique (EST)

Akagami Koji, Nakamura Ryuta, Nanjo Hiroshi, Minamiya Yoshihiro

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2018S ( 0 ) 481 - 482   2018.03

DOI CiNii Research

Study on transpiration suppression of reagents for applying electric field stirring technique (EST) to rapid in situ hybridization (part.2)

Nakamura Ryuta, Akagami Koji, Fujishima Satoshi, Kusumi Takayuki, Nanjo Hiroshi, Minamiya Yoshihiro, Akagami Yoichi

Proceedings of JSPE Semestrial Meeting ( The Japan Society for Precision Engineering )  2018S ( 0 ) 483 - 484   2018.03

DOI CiNii Research