Research Achievements - Original paper -
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Activation of TRPV4 channels(hVRL2/mTRP12) by phorbol derivatives.
Watanabe H et. Al
J Biol Chem 277 ( 16 ) 13569 - 13577 2002.01 [Refereed]
Research paper (journal) Domestic Co-author
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Heat-evoked activation of TRPV4 channels in a HEK293 cell expression system and in native mouse aorta endothelial cells.
Watanabe H et. al
J Biol Chem 277 ( 49 ) 47044 - 47051 2002.01 [Refereed]
Research paper (journal) Domestic Co-author
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CaT1 and the Calcium Release-activated Calcium Channel Manifest Distinct Pore Properties
Thomas Voets, Jean Prenen, Andrea Fleig, Rudi Vennekens, Hiroyuki Watanabe, Joost G.J. Hoenderop,René J.M. Bindels,Guy Droogmans, Reinhold Penner, Bernd Nilius
JOURNAL OF BIOLOGICAL CHEMISTRY 276 ( 51 ) 47767 - 47770 2001.12 [Refereed]
Research paper (journal)
The calcium release-activated calcium channel (CRAC) is a highly Ca2+-selective ion channel that is activated on depletion of inositol triphosphate (IP3)-sensitive intracellular Ca2+ stores. It was recently reported that CaT1, a member of the TRP family of cation channels, exhibits the unique biophysical properties of CRAC, which led to the conclusion that CaT1 comprises all or part of the CRAC pore (Yue, L., Peng, J. B., Hediger, M. A., and Clapham, D. E. (2001) Nature 410, 705–709). Here, we directly compare endogenous CRAC with heterologously expressed CaT1 and show that they manifest several clearly distinct properties. CaT1 can be distinguished from CRAC in the following features: sensitivity to store-depleting agents; inward rectification in the absence of divalent cations; relative permeability to Na+ and Cs+; effect of 2-aminoethoxydiphenyl borate (2-APB). Moreover, CaT1 displays a mode of voltage-dependent gating that is fully absent in CRAC and originates from the voltage-dependent binding/unbinding of Mg2+ inside the channel pore. Our results imply that the pores of CaT1 and CRAC are not identical and indicate that CaT1 is a Mg2+-gated channel not directly related to CRAC.
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Congestive Heart Failure Associated with Portasystemic Shunt by Small-Bowel Varices
KIBIRA Satoshi, ISHIDA Hideaki, WATANABE Hiroyuki, IZUMI Chikako, OOMOTO Naoki, OGAWA Yasuhiko, SAITO Takashi, MIURA Mamoru
日本臨床生理学会雑誌 30 ( 1 ) 59 - 64 2000.02 [Refereed]
Research paper (journal)
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Validity of the right ventricular Doppler index for assessment of severity of congestive heart failure in patients with dilated cardiomyopathy
C Izumi
Heart and vessels 14 ( 5 ) 232 - 239 1999.09 [Refereed]
Research paper (journal)
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Calcium channel blocking properties of NKY-722, a highly hydrophilic dihydropyridine derivative, in single ventricular cells of guinea-pig hearts
Watanabe H, Nishio M, Iijima T
Japanese Heart Journal 35 451 - 452 1994 [Refereed]
Research paper (journal)