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Affiliation |
Director/Vice President |
Research Interests 【 display / non-display 】
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自動能
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循環
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心臓
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電気生理学・生理学
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細胞生理学
Graduating School 【 display / non-display 】
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-1983.03
Kyushu University Faculty of Medicine Graduated
Campus Career 【 display / non-display 】
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2009.04-2022.03
Akita University Graduate School of Medicine Doctorial Course in Medicine Bioregulatory Medicine Department of Cell Physiology Professor
Research Areas 【 display / non-display 】
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Life Science / Physiology
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Life Science / Pharmacology
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Life Science / Biomedical engineering
Thesis for a degree 【 display / non-display 】
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Calcium-activated non-selective cation channel in ventricular cells isolated from adult guinea-pig hearts.
Ehara T, Noma A, Ono K
Journal of Physiology 403 117 - 133 1990.02 [Refereed]
Domestic Co-author
Research Achievements 【 display / non-display 】
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Comparative Study of Transcriptome in the Hearts Isolated from Mice, Rats, and Humans
Okada D.
Biomolecules ( Biomolecules ) 12 ( 6 ) 2022.06 [Refereed]
Research paper (journal)
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Okamoto Y.
Biomolecules ( Biomolecules ) 12 ( 5 ) 2022.05 [Refereed]
Research paper (journal)
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Nomura K.
Environmental Health and Preventive Medicine ( Environmental Health and Preventive Medicine ) 26 ( 1 ) 2021.12 [Refereed]
Research paper (journal)
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Fukuda Y.
Biology of Reproduction ( Biology of Reproduction ) 105 ( 1 ) 258 - 266 2021.07 [Refereed]
Research paper (journal)
<jats:title>Abstract</jats:title>
<jats:p>To cryopreserve cells, it is essential to avoid intracellular ice formation during cooling and warming. One way to achieve this is to convert the water inside the cells into a non-crystalline glass. It is currently believed that to accomplish this vitrification, the cells must be suspended in a very high concentration (20–40%) of a glass-inducing solute, and subsequently cooled very rapidly. Herein, we report that this belief is erroneous with respect to the vitrification of one-cell rat embryos. In the present study, one-cell rat embryos were vitrified with 5 μL of EFS10 (a mixture of 10% ethylene glycol (EG), 27% Ficoll, and 0.45 M sucrose) in cryotubes at a moderate cooling rate, and warmed at various rates. Survival was assessed according to the ability of the cells to develop into blastocysts and to develop to term. When embryos were vitrified at a 2613 °C/min cooling rate and thawed by adding 1 mL of sucrose solution (0.3 M, 50 °C) at a warming rate of 18 467 °C/min, 58.1 ± 3.5% of the EFS10-vitrified embryos developed into blastocysts, and 50.0 ± 4.7% developed to term. These rates were similar to those of non-treated intact embryos. Using a conventional cryotube, we achieved developmental capabilities in one-cell rat embryos by rapid warming that were comparable to those of intact embryos, even using low concentrations (10%) of cell-permeating cryoprotectant and at low cooling rates.</jats:p> -
Takahashi K.
Heliyon ( Heliyon ) 7 ( 6 ) 2021.06 [Refereed]
Research paper (journal)
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Comparative Study of Transcriptome in the Hearts Isolated from Mice, Rats, and Humans
Okamoto Yosuke, Okada Daigo, Kobayashi Daiki, Ono Kyoichi, Ishii Kuniaki
Proceedings for Annual Meeting of The Japanese Pharmacological Society ( Japanese Pharmacological Society ) 96 ( 0 ) 3-B-P-217 2022
<p>The heart is a critical organ for maintaining life in mammals and has long been one of the most important targets of scientific research, and the basic molecular mechanisms of heart beat appear to have already been established. However, few studies have focused on species differences, and challenges remain in studying genes that have universal functions across species and genes that determine species differences. Here, we analyzed transcriptome data from mouse, rat, and human atria, ventricles, and sinus node (SA) and calculated and compared specificity measure (SPM) values that account for species differences among the three cardiac regions. SA has the largest species differences and we searched for a gene, which by our criteria was SHOX2; the SPM value for SHOX2 was prominently high across species. Similarly, SPM values identified 3 atrial-specific markers, 11 ventricular-specific markers, and 17 SA-specific markers. Ontology analysis identified 70 cardiac region- and species-specific ontologies. These results suggest that reanalysis of existing data by calculating SPM values may identify novel tissue-specific and species-dependent gene expression. This study demonstrates that SHOX2 is an SA-specific transcription factor, a novel cardiac region marker, and that species-dependent ontology is important. No COI.</p>
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Association of stay-home order during COVID-19 pandemic with depressive symptoms and suicide-related ideation in university students in Japan
野村恭子, 南園佐知子, 前田恵理, KIM Roseline, 岩田豊人, 平山純子, 尾野恭一, 伏見雅人, 後藤猛, 三島和夫, 山本文雄
日本心身医学会総会ならびに学術講演会抄録集 62nd (CD-ROM) 2021
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Impact of hyperpolarization-activated Cl- current on arrhythmogenecity of rat pulmonary vein cardiomyocytes
Okamoto Yosuke, Ono Kyoichi, Takano Makoto, Ishii Kuniaki
JOURNAL OF PHARMACOLOGICAL SCIENCES 130 ( 3 ) S77 2016.03
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BREATHING RHYTHM STABILIZATION IN NEWBORN MICE UNDER RESTRAINT STRESS INDUCED BY ECG ELECTRODE ATTACHMENT
S. Sato, T. Kanbayashi, H. Kondo, J. Tokunaga, Y. Sagawa, M. Sato, K. Hosokawa, K. Ono, T. Shimizu
SLEEP ( AMER ACAD SLEEP MEDICINE ) 33 A21 - A21 2010
Summary of the papers read (international conference)
◆Original paper【 display / non-display 】
◆Other【 display / non-display 】
Grant-in-Aid for Scientific Research 【 display / non-display 】
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Grant-in-Aid for Scientific Research(C)
Project Year： 2022.04 - 2025.03
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Investigation of mechanisms of pain evoked by meteorological environment
Grant-in-Aid for Challenging Research (Pioneering)
Project Year： 2020.04 - 2022.03
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Grant-in-Aid for Scientific Research(C)
Project Year： 2018.04 - 2021.03
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Mechanisms of temperature- and pressure-induced pain
Challenging Research (Pioneering)
Project Year： 2017.06 - 2021.03
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Voltage gated sodium channel-related hereditary pain diseases
Grant-in-Aid for Scientific Research(C)
Project Year： 2017.04 - 2020.03 Investigator(s): TAKAHASHI TSUTOMU