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Affiliation |
Director/Vice President |
Research Interests 【 display / non-display 】
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自動能
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循環
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心臓
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電気生理学・生理学
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細胞生理学
Graduating School 【 display / non-display 】
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-1983.03
Kyushu University Faculty of Medicine Graduated
Campus Career 【 display / non-display 】
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2009.04-2022.03
Akita University Graduate School of Medicine Doctorial Course in Medicine Bioregulatory Medicine Department of Cell Physiology Professor
Thesis for a degree 【 display / non-display 】
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Calcium-activated non-selective cation channel in ventricular cells isolated from adult guinea-pig hearts.
Ehara T, Noma A, Ono K
Journal of Physiology 403 117 - 133 1990.02 [Refereed]
Domestic Co-author
Research Achievements 【 display / non-display 】
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Comparative Study of Transcriptome in the Hearts Isolated from Mice, Rats, and Humans
Okada D.
Biomolecules ( Biomolecules ) 12 ( 6 ) 2022.06 [Refereed]
Research paper (journal)
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Okamoto Y.
Biomolecules ( Biomolecules ) 12 ( 5 ) 2022.05 [Refereed]
Research paper (journal)
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Nomura K.
Environmental Health and Preventive Medicine ( Environmental Health and Preventive Medicine ) 26 ( 1 ) 2021.12 [Refereed]
Research paper (journal)
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Fukuda Y.
Biology of Reproduction ( Biology of Reproduction ) 105 ( 1 ) 258 - 266 2021.07 [Refereed]
Research paper (journal)
<jats:title>Abstract</jats:title>
<jats:p>To cryopreserve cells, it is essential to avoid intracellular ice formation during cooling and warming. One way to achieve this is to convert the water inside the cells into a non-crystalline glass. It is currently believed that to accomplish this vitrification, the cells must be suspended in a very high concentration (20–40%) of a glass-inducing solute, and subsequently cooled very rapidly. Herein, we report that this belief is erroneous with respect to the vitrification of one-cell rat embryos. In the present study, one-cell rat embryos were vitrified with 5 μL of EFS10 (a mixture of 10% ethylene glycol (EG), 27% Ficoll, and 0.45 M sucrose) in cryotubes at a moderate cooling rate, and warmed at various rates. Survival was assessed according to the ability of the cells to develop into blastocysts and to develop to term. When embryos were vitrified at a 2613 °C/min cooling rate and thawed by adding 1 mL of sucrose solution (0.3 M, 50 °C) at a warming rate of 18 467 °C/min, 58.1 ± 3.5% of the EFS10-vitrified embryos developed into blastocysts, and 50.0 ± 4.7% developed to term. These rates were similar to those of non-treated intact embryos. Using a conventional cryotube, we achieved developmental capabilities in one-cell rat embryos by rapid warming that were comparable to those of intact embryos, even using low concentrations (10%) of cell-permeating cryoprotectant and at low cooling rates.</jats:p> -
Takahashi K.
Heliyon ( Heliyon ) 7 ( 6 ) 2021.06 [Refereed]
Research paper (journal)