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大学院医学系研究科（医学専攻等） 医学専攻 病態制御医学系 総合診療・検査診断学講座 |
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秋田市本道１-１-１ 臨床南棟4F |
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018-884-6209 |
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植木 重治 (ウエキ シゲハル)
UEKI Shigeharu
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職務経歴（学内） 【 表示 ／ 非表示 】
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2021年10月-継続中
秋田大学 大学院医学系研究科（医学専攻等） 医学専攻 病態制御医学系 総合診療・検査診断学講座 教授
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2014年04月-2021年09月
秋田大学 大学院医学系研究科（医学専攻等） 医学専攻 病態制御医学系 総合診療・検査診断学講座 准教授
研究等業績 【 表示 ／ 非表示 】
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A case of eosinophilic granulomatosis with polyangiitis preceded by allergic bullous lesions
Hasegawa E.
Modern Rheumatology Case Reports ( Modern Rheumatology Case Reports ) 9 ( 1 ) 143 - 148 2025年01月
研究論文（学術雑誌）
A 62-year-old man with a history of diabetes mellitus was hospitalised with numbness of lower limbs, bullous lesions of the whole body, kidney dysfunction, presence of eosinophils, and elevated antineutrophil cytoplasmic antibodies to myeloperoxidase and anti-bullous pemphigoid 180 antibodies and was diagnosed with mononeuritis multiplex. Kidney and muscle biopsies showed vasculitis with fibrinoid necrosis, whereas skin biopsies showed only blister formation between the epidermis and dermis; a high eosinophilic infiltrate was present in all three tissues. These findings led to a diagnosis of eosinophilic granulomatosis with polyangiitis combined with allergic bullous lesions. Immunohistological examination indicated cytolytic eosinophils and extracellular traps, suggesting the presence of eosinophil extracellular trap cell death (eosinophil ETosis) in diseased tissue.
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Miyabe Y.
Journal of Allergy and Clinical Immunology ( Journal of Allergy and Clinical Immunology ) 2024年
研究論文（学術雑誌）
BACKGROUND: Airway obstruction caused by viscous mucus is an important pathophysiological characteristic of persistent inflammation, which can result in organ damage. OBJECTIVE: We investigated the hypothesis that the biophysical characteristics of accumulating granulocytes impact the clinical properties of mucus. METHODS: Surgically acquired nasal mucus samples from patients with eosinophilic chronic rhinosinusitis and neutrophil-dominant, non-eosinophilic chronic rhinosinusitis were evaluated in terms of computed tomography density, viscosity, water content, wettability, and protein composition. Isolated human eosinophils and neutrophils were stimulated to induce the formation of extracellular traps, followed by the formation of aggregates. The biophysical properties of the aggregated cells were also examined. RESULTS: Mucus from patients with eosinophilic chronic rhinosinusitis had significantly higher computed tomography density, viscosity, dry weight, and hydrophobicity compared with mucus from patients with non-eosinophilic chronic rhinosinusitis. The levels of eosinophil-specific proteins in mucus correlated with its physical properties. Eosinophil and neutrophil aggregates showed physical and pathological characteristics resembling those of mucus. Co-treatment with deoxyribonuclease and heparin, which slenderizes the structure of eosinophil extracellular traps, efficiently induced reductions in the viscosity and hydrophobicity of both eosinophil aggregates and eosinophilic mucus. CONCLUSIONS: The present study elucidates the pathogenesis of mucus stasis in infiltrated granulocyte aggregates from a novel perspective. These findings may contribute to the development of treatment strategies for eosinophilic airway diseases.
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Rapidly progressive mucus plugs in allergic bronchopulmonary mycosis
Miyazaki O.
Journal of Asthma ( Journal of Asthma ) 1 - 9 2024年
研究論文（学術雑誌）
INTRODUCTION: Allergic bronchopulmonary mycosis (ABPM) is a chronic airway disease characterized by the presence of fungi that trigger allergic reactions and airway obstruction. Here, we present a unique case of ABPM in which a patient experienced sudden respiratory failure due to mucus plug-induced airway obstruction. The patient's life was saved by venovenous extracorporeal membrane oxygenation (VV-ECMO) and bronchoscopic removal of the plug. This case emphasizes the clinical significance of mucus plug-induced airway obstruction in the differential diagnosis of respiratory failure in patients with ABPM. CASE STUDY: A 52-year-old female clerical worker with no smoking history, presented with dyspnea. CT scan revealed mucus plugs in both lungs. Despite treatment, the dyspnea progressed rapidly to respiratory failure, leading to VV-ECMO placement. RESULTS: CT revealed bronchial wall thickening, obstruction, and extensive atelectasis. Bronchoscopy revealed extensive mucus plugs that were successfully removed within two days. The patient's respiratory status significantly improved. Follow-up CT revealed no recurrence. Fungal cultures identified Schizophyllum commune, confirming ABPM. Histological examination of the mucus plugs revealed aggregated eosinophils, eosinophil granules, and Charcot-Leyden crystals. Galectin-10 and major basic protein (MBP) staining supported these findings. Eosinophil extracellular traps (EETs) and eosinophil cell death (ETosis), which contribute to mucus plug formation, were identified by citrullinated histone H3 staining. CONCLUSION: Differentiating between asthma exacerbation and mucus plug-induced airway obstruction in patients with ABPM and those with acute respiratory failure is challenging. Prompt evaluation of mucous plugs and atelectasis using CT and timely decision to introduce ECMO and bronchoscopic mucous plug removal are required.
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Sato T.
Journal of Dermatological Science ( Journal of Dermatological Science ) 112 ( 1 ) 6 - 14 2023年10月
研究論文（学術雑誌）
BACKGROUND: Bullous pemphigoid (BP) is an autoimmune bullous disease in which abundant eosinophils accumulate in the blisters. Galectin-10 abounds in the cytoplasm of eosinophils and is released as a result of eosinophil extracellular trap cell death (EETosis). OBJECTIVE: To identify EETosis and the pathological roles of galectin-10 in BP. METHODS: EETosis and galectin-10 in BP blisters were confirmed by immunofluorescence and transmission electron microscopy. The concentrations of galectin-10 in serum and blister fluid from BP patients were studied by ELISA. The matrix metalloproteinase (MMP) expression in BP blisters was immunohistochemically compared to that in healthy controls. As an in vitro assay, normal human epidermal keratinocytes (NHEKs) and normal human dermal fibroblasts (NHDFs) were stimulated with galectin-10, followed by MMP expression measurement by real-time PCR and ELISA. The signaling pathways activated by galectin-10 were studied using Western blotting and confirmed by inhibition assays. RESULTS: Galectin-10-containing eosinophil infiltration and the extracellular deposition of major basic protein were observed in BP blisters. The ultrastructural characteristics of tissue eosinophils indicated piecemeal degranulation and EETosis. In the BP patients, the concentration of galectin-10 was higher in the blister fluid than in the serum. Several types of MMPs were upregulated in BP blisters. Galectin-10 upregulated the production of MMPs through the pathways of p38 MAPK, ERK and JNK in NHEKs and NHDFs. CONCLUSION: In the BP blisters, the eosinophils underwent EETosis and released galectin-10. Galectin-10 might contribute to BP blister formation through the production of MMPs by keratinocytes and fibroblasts.
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Allergic fungal rhinosinusitis: What we can learn from allergic bronchopulmonary mycosis
Nakayama T.
Allergology International ( Allergology International ) 72 ( 4 ) 521 - 529 2023年10月
研究論文（学術雑誌）
<p>Allergic fungal rhinosinusitis (AFRS) and allergic bronchopulmonary mycosis (ABPM) are inflammatory disorders of the respiratory tract resulting from type 1 and 3 hypersensitivity reactions against fungi. The hallmark features of both diseases are eosinophil infiltration into the airway mucosa caused by localized type 2 inflammation and concomitant viscid secretions in the airways. Eosinophilic mucin-induced compression of adjacent anatomic structures leads to bone erosion and central bronchiectasis in the upper and lower respiratory tracts, respectively. Although these diseases share common features in their pathogenesis, they also exhibit notable differences. Epidemiologic findings are diverse, with AFRS typically presenting at a younger age, exhibiting less complicated bronchial asthma, and displaying lower total immunoglobulin E levels in laboratory findings compared with ABPM. Furthermore, despite their similar pathogenesis, the rarity of sinio-bronchial allergic mycosis in both AFRS and ABPM underscores the distinctions between these two diseases. This review aims to clarify the similarities and differences in the pathogenesis of AFRS and ABPM to determine what can be learned about AFRS from ABPM, where more is known.</p>
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Onoue M.
Allergology International ( Allergology International ) 73 ( 1 ) 177 - 179 2024年01月
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Through the MIRRA and what we found there
Ueki S.
ERJ Open Research ( ERJ Open Research ) 10 ( 1 ) 2024年01月
A series of post hoc MIRRA studies have illuminated eosinophilic granulomatosis with polyangiitis as an eosinophil-driven disease from various perspectives https://bit.ly/468pj86.
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Fungi and immune response: An update
Ueki S.
Allergology International ( Allergology International ) 72 ( 4 ) 491 - 492 2023年10月
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喘息の周辺病態 アレルギー性気管支肺真菌症における好酸球の表面抗原の網羅的解析
佐々木 寿, 宮田 純, 松山 笑子, 砂田 啓英也, 正木 克宜, 加畑 宏樹, 川名 明彦, 植木 重治, 浅野 浩一郎, 福永 興壱
アレルギー ( (一社)日本アレルギー学会 ) 72 ( 6-7 ) 910 - 910 2023年08月
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気管支喘息(成人):病態生理 喘息患者のFEV1経年低下予測因子としての血清Galectin-10値の有用性
小林 このみ, 長瀬 洋之, 岩永 賢司, 田中 明彦, 原田 紀宏, 辻口 博聖, 増子 裕典, 斎藤 純平, 鈴川 真穂, 町田 健太朗, 植木 重治, 相良 博典, 檜澤 伸之, 井上 博雅, 中村 裕之, 東田 有智, 大田 健
アレルギー ( (一社)日本アレルギー学会 ) 72 ( 6-7 ) 866 - 866 2023年08月
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抗血小板粘着性を示す生分解性共重合体
特許
特願 特願2019-174742 特開 特開2020-056020
出願日： 2019年09月25日
公開日： 2020年04月09日
寺境 光俊, 松本 和也, 廣川 誠, 植木 重治, 柏谷 啓太, 竹田 麻央, 工藤 滉平, 福岡 玲, 疋田 正喜, 齋藤 希望
科研費（文科省・学振）獲得実績 【 表示 ／ 非表示 】
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重症インフルエンザ肺炎におけるウイルス特異的応答と好酸球死誘導
基盤研究(C)
研究期間: 2022年04月 - 2025年03月 代表者: 宮入 烈, 植木 重治
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マイクロファイバー細胞分離を用いた消化管アレルギーとその関連疾患の病態解明
基盤研究(C)
研究期間: 2021年04月 - 2024年03月 代表者: 山田 佳之, 林 泰秀, 加藤 政彦, 高井 まどか, 植木 重治
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マイクロファイバー細胞分離を用いた消化管アレルギーとその関連疾患の病態解明
基盤研究(C)
研究期間: 2021年04月 - 2024年03月 代表者: 山田 佳之, 林 泰秀, 加藤 政彦, 高井 まどか, 植木 重治
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喘息病態における好酸球ETosisと気道上皮細胞の相互作用に関する分子生物学検討
基盤研究(C)
研究期間: 2021年04月 - 2024年03月 代表者: 竹田 正秀, 植木 重治
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マイクロファイバー細胞分離を用いた消化管アレルギーとその関連疾患の病態解明
基盤研究(C)
研究期間: 2021年04月 - 2024年03月 代表者: 山田 佳之, 林 泰秀, 加藤 政彦, 高井 まどか, 植木 重治