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Affiliation |
Graduate School of Medicine Doctorial Course in Medicine Bioregulatory Medicine Department of Cell Biology and Morphology |
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Date of Birth |
1983 |
YONEMURA Yoji
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Research Interests 【 display / non-display 】
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Alzheimer's disease
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タンパク質の局在
Graduating School 【 display / non-display 】
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2005.04-2009.03
Waseda University Graduated
Graduate School 【 display / non-display 】
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2011.04-2014.03
The University of Tokyo Graduate School, Division of General Culture Doctor's Course Completed
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2009.04-2011.03
The University of Tokyo Graduate School, Division of General Culture Master's Course Completed
Studying abroad experiences 【 display / non-display 】
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2015.04-2019.03
Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) Postdoctoral Fellow
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2014.04-2015.03
Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) JSPS Research Fellowship for Young Scientists (PD)
Campus Career 【 display / non-display 】
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2025.12-Now
Akita University Graduate School of Medicine Doctorial Course in Medicine Bioregulatory Medicine Department of Cell Biology and Morphology Assistant Professor
External Career 【 display / non-display 】
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2025.12
Akita University Graduate School of Medicine Assistant Professor
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2024.06-2025.11
Kobe University Engineering Biology Research Center
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2019.04-2024.03
Doshisha University Organization for Research Initiatives and Development Assistant Professor
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2015.04-2019.03
Leibniz Institut für Altersforschung, Fritz-Lipmann-Institut (FLI)
Research Areas 【 display / non-display 】
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Life Science / Cell biology
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Life Science / Cell biology
Research Achievements 【 display / non-display 】
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A YIPF5-GOT1A/B complex directs a transcription independent function of ATF6 in ER export
Paul Cramer , Yoji Yonemura , Laura Behrendt , Aleksandra Marszalek , Mara Sannai , William Durso , Cagatay Günes , Karol Szafranski , Nobuhiro Nakamura , Tornike Nasrashvili , Johanna Mayer , Björn von Eyss, Christoph Kaether
bioRxiv : the preprint server for biology 2023.12
Research paper (journal) International Co-author
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Active Transport by Cytoplasmic Dynein Maintains the Localization of MAP2 in Developing Neurons.
Yoji Yonemura, Yuri Sakai, Rinaho Nakata, Ayaka Hagita-Tatsumoto, Tomohiro Miyasaka, Hiroaki Misonou
bioRxiv : the preprint server for biology 2023.04
Research paper (journal) Domestic Co-author
MAP2 has been widely used as a marker of neuronal dendrites because of its extensive restriction in the somatodendritic region of neurons. Despite that, how the precise localization of such a soluble protein is established and maintained against thermal forces and diffusion has been elusive and long remained a mystery in neuroscience. In this study, we aimed to uncover the mechanism behind how MAP2 is retained in the somatodendritic region. Using GFP-tagged MAP2 expressed in cultured hippocampal neurons, we discovered a crucial protein region responsible for the localization of MAP2, the serine/proline-rich (S/P) region. Our pulse-chase live-cell imaging revealed the slow but steady migration of MAP2 toward distal dendrites, which was not observed in a MAP2 mutant lacking the S/P region, indicating that S/P-dependent transport is vital for the proper localization of MAP2. Furthermore, our experiments using an inhibitor of cytoplasmic Dynein, ciliobrevin D, as well as Dynein knockdown, showed that cytoplasmic Dynein is involved in the transport of MAP2 in dendrites. We also found that Dynein complex binds to MAP2 through the S/P region in heterologous cells. Using mathematical modeling based on experimental data, we confirmed that an intermittent active transport mechanism is essential. Thus, we propose that the cytoplasmic Dynein recruits and transports free MAP2 toward distal dendrites, thereby maintaining the precise dendritic localization of MAP2 in neurons. Our findings shed light on the previously unknown mechanism behind MAP2 localization and provide a new direction for soluble protein trafficking research in the field of cell biology of neurons.
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COPII collar defines the boundary between ER and ER exit site and does not coat cargo containers.
Olga Shomron, Inbar Nevo-Yassaf, Tamar Aviad, Yakey Yaffe, Eitan Erez Zahavi, Anna Dukhovny, Eran Perlson, Ilya Brodsky, Adva Yeheskel, Metsada Pasmanik-Chor, Anna Mironov, Galina V Beznoussenko, Alexander A Mironov, Ella H Sklan, George H Patterson, Yoji Yonemura, Mara Sannai, Christoph Kaether, Koret Hirschberg
The Journal of cell biology 220 ( 6 ) 2021.06 [Refereed]
Research paper (journal) International Co-author
COPII and COPI mediate the formation of membrane vesicles translocating in opposite directions within the secretory pathway. Live-cell and electron microscopy revealed a novel mode of function for COPII during cargo export from the ER. COPII is recruited to membranes defining the boundary between the ER and ER exit sites, facilitating selective cargo concentration. Using direct observation of living cells, we monitored cargo selection processes, accumulation, and fission of COPII-free ERES membranes. CRISPR/Cas12a tagging, the RUSH system, and pharmaceutical and genetic perturbations of ER-Golgi transport demonstrated that the COPII coat remains bound to the ER-ERES boundary during protein export. Manipulation of the cargo-binding domain in COPII Sec24B prohibits cargo accumulation in ERES. These findings suggest a role for COPII in selecting and concentrating exported cargo rather than coating Golgi-bound carriers. These findings transform our understanding of coat proteins' role in ER-to-Golgi transport.
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Inhibition of cargo export at ER exit sites and the trans-Golgi network by the secretion inhibitor FLI-06
Yoji Yonemura, Xiaolin Li, Katja Mueller, Andreas Kraemer, Paul Atigbire, Torben Mentrup, Talitha Feuerhake, Torsten Kroll, Olga Shomron, Richard Nohl, Hans-Dieter Arndt, Christian Hoischen, Peter Hemmerich, Koret Hirschberg, Christoph Kaether
JOURNAL OF CELL SCIENCE 2016.10 [Refereed]
Research paper (journal) International Co-author
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Specific combinations of presenilins and Aph1s affect the substrate specificity and activity of γ-secretase.
Yoji Yonemura, Eugene Futai, Sosuke Yagishita, Christoph Kaether, Shoichi Ishiura
Biochemical and biophysical research communications 2016.09 [Refereed]
Research paper (journal) International Co-author
◆Original paper【 display / non-display 】
Grant-in-Aid for Scientific Research 【 display / non-display 】
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Elucidation of the mechanism underlying the somatodendritic specific localization of microtubule associated protein 2 (MAP2)
Grant-in-Aid for Early-Career Scientists
Project Year： 2025.04 - 2028.03
Presentations 【 display / non-display 】
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Active transport by cytoplasmic dynein regulates somatodendritic localization of MAP2
Yoji Yonemura, Yuri Sakai, Tomohiro Miyasaka, Hiroaki Misonou
第98回日本生化学会大会 (京都) 2025.11 - 2025.11 日本生化学会
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MAP2の適切な局在化に関与する因子の同定
米村洋而, 酒井宥璃, 萩田彩香, 中田, 里奈穂, 宮坂知宏, 御園生裕明
第44回日本神経科学大会 / CJK第1回国際会議 2021.07 - 2021.07
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MAP2の局在を決定する領域の同定
米村洋而, 宮坂知宏, 御園生裕明
第43回日本神経科学大会 2020.07 - 2020.07
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The COPII heterocomplex is a gatekeeper that selects and concentrates transportcompetent cargo in endoplasmic reticulum exit sites
Koret Hirschberg, Olga Shomron, Inbar Nevo-Yassaf, Eran Perlson, George H. Patterson, Yoji Yonemura, Christoph Kaether
EMBO Workshop on “ER function in health and disease” 2018.10 - 2018.10
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Genome-wide loss- and gain-of-function screens to find novel players in ER-export
Yoji Yonemura, Laura Behrendt, Mara Sannai, Paul Cramer, Björn von Eyß, Christoph Kaether
EMBO Workshop on “ER function in health and disease” 2018.10 - 2018.10