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Affiliation |
Graduate School of Medicine Advanced Research Center for Geriatric Medicine |
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Laboratory Address |
3F, Research building for Basic Medicine, 1-1-1, Hondo, Akita 010-8543 JAPAN |
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Mail Address |
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Special Affairs |
MAYANAGI Takako |
OHNUMA Takako
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Graduating School 【 display / non-display 】
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1994.04-1998.03
Iwate University Faculty of Agriculture Graduated
Graduate School 【 display / non-display 】
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2000.04-2003.03
Iwate University Graduate School, Division of Agricltural Sciences Doctor's Course Completed
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1998.04-2000.03
Iwate University Graduate School, Division of Agriculture Master's Course Completed
Campus Career 【 display / non-display 】
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2025.04-Now
Akita University Graduate School of Medicine Advanced Research Center for Geriatric Medicine Specially-appointed Assistant Professor
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2024.07-2025.03
Akita University Advanced Research Center for Geriatric Medicine Specially-appointed Assistant Professor
Research Areas 【 display / non-display 】
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Life Science / Laboratory animal science
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Life Science / Molecular biology
Research Achievements 【 display / non-display 】
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Hidetaka Ota* , Takako Ohnuma , Ayuto Kodama , Tatsunori Shimizu , Kaoru Sugawara and Fumio Yamamoto
Cells ( MDPI ) 14 340 2025.02 [Refereed]
Research paper (journal) Domestic Co-author
Ageing is a major risk factor for cognitive and physical decline, but its mechanisms remain poorly understood. This study aimed to detect early cognitive and physical changes, and to analyze the pathway involved by monitoring two groups of mice: a young and an adult group. The study has identified the types of molecules involved in the hippocampus. Adult mice (47 weeks) showed significantly reduced exploratory behavior compared to young mice (11 weeks), although spatial working memory showed no difference. In terms of physical function, grip strength was significantly reduced in adult mice. The Frailty Index (FI) further highlighted age-related changes in adult mice. To investigate the causes of cognitive decline, adult mice were categorized based on their declining cognitive function. Microarray analysis of their hippocampi revealed that the cholinergic receptor nicotinic α3 subunit (Chrna3) was significantly reduced in mice with cognitive decline compared to controls. Subsequent in vitro experiments showed that oxidative stress and cholinesterase inhibitors decreased Chrna3 expression, whereas nicotine and cytisine increased it. These results suggest that Chrna3 is a key factor in age-related cognitive decline. The development of therapeutic strategies targeting Chrna3 expression may offer promising avenues for preclinical and clinical research to mitigate cognitive ageing.
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Tatsunori Shimizu, Hidetaka Ota, Ayuto Kodama, Yasuhiro Suzuki, Takako Ohnuma, Rieko Suzuki, Kaoru Sugawara, Yasushi Sato, Hiroyuki Kodama
Biomolecules ( MDPI ) 14 ( 9 ) 2024.08 [Refereed]
Research paper (journal) Domestic Co-author
With aging populations in many countries, including Japan, efforts to mitigate the aging-related decline in physical function have gained importance not only for improving individual quality of life but also for mitigating the effects of this loss of function on society. Impaired glucose tolerance, muscle weakness, and cognitive decline are well-known effects of aging. These interrelated factors can create a vicious cycle because impaired glucose tolerance can accelerate muscle weakness and cognitive decline. Unmodulated 40 Hz (u40Hz) stimulation is imperceptible to the human ear and has been reported to improve cognitive function in humans and mice. However, research on the effects of u40Hz stimulation is still limited. This study aimed to report the effects of u40Hz stimulation on glucose tolerance and muscle strength in senescence-accelerated prone (SAMP)-10 mice, a model of accelerated aging. SAMP-10 mice underwent five weeks of u40Hz stimulation followed by glucose-tolerance tests, cognitive and behavioral assessments, and frailty evaluations. In comparison with the control group, the u40Hz-stimulation group showed mitigation of age-related decline in glucose tolerance, a better frailty index (FI), and notably preserved muscle strength. Microarray analysis of stimulated muscle tissue revealed significant upregulation of β-oxidation genes and genes functioning downstream of peroxisome proliferator-activated receptor gamma, and significant downregulation of clock genes. These findings indicate the beneficial effects of u40Hz stimulation on glucose tolerance, muscle strength, and cognitive function, warranting further research in this area.
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Molecular spectrum of somaclonal variation in regenerated rice revealed by whole-genome sequencing
Akio Miyao, Mario Nakagome, takako Ohnuma, Harumi Ymagata, hiroyuki kanamori, Yuichi Katayose, Akira Takahashi, Takashi Matsumoto, Hirohiko Hirochika
Plant & Cell physiology ( Oxford academic ) 53 ( 1 ) 256 - 264 2012.01 [Refereed]
Research paper (journal) Domestic Co-author
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Association of culture of mouse urogenital complexes in media containing rodent sera with the appearance of primordial germ cell-like cells.
T.Mayanagi, K.Ito, J,Takahashi
Reproduction ( Bioscientifia Ltd ) ( 125 ) 519 - 526 2003.01 [Refereed]
Research paper (journal) Single author
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Purification of mouse primordial germ cells by Nycodenz
T.Mayanagi, R.Kurosawa, K.Ohnuma, K.Ito, J,Takahashi
Reproduction ( Bioscientifia Ltd ) ( 125 ) 667 - 675 2003.01 [Refereed]
Research paper (journal) Single author