NAGAKI Yushi

写真a

Affiliation

Graduate School of Medicine  Doctorial Course in Medicine  Oncoregulatory Medicine  Department of Thoracis Surgery

Date of Birth

1987
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Research Interests 【 display / non-display 】

  • リンパ節転移

  • 転移

  • 癌

  • 消化器癌

  • 食道癌

display all >>

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Graduating School 【 display / non-display 】

  • 2006.04
    -
    2012.03

    Akita University   Faculty of Medicine   Graduated

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Graduate School 【 display / non-display 】

  • 2014.04
    -
    2020.09

    Akita University  Graduate School, Division of Medicine  Doctor's Course  Completed

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Campus Career 【 display / non-display 】

  • 2022.07
    -
    Now

    Akita University   Graduate School of Medicine   Doctorial Course in Medicine   Oncoregulatory Medicine   Department of Thoracis Surgery   Assistant Professor  

  • 2017.04
    -
    2021.03

    Akita University   Graduate School of Medicine   Doctorial Course in Medicine   Oncoregulatory Medicine   Department of Thoracis Surgery   Graduate Student  

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Qualification acquired 【 display / non-display 】

  • Doctor

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Thesis for a degree 【 display / non-display 】

  • m6A demethylase ALKBH5 promotes proliferation of esophageal squamous cell carcinoma associated with poor prognosis.

    Yushi Nagaki, Satoru Motoyama, Tomokazu Yamaguchi, Midori Hoshizaki, Yusuke Sato, Teruki Sato, Yukio Koizumi, Akiyuki Wakita, Yuta Kawakita, Kazuhiro Imai, Hiroshi Nanjo, Hiroyuki Watanabe, Yumiko Imai, Yoshihiro Minamiya, Keiji Kuba 

    Genes to cells    2020.09  [Refereed]

    Single author

    Esophageal squamous cell carcinoma (ESCC) is one of the most fatal types of malignant tumors worldwide. Epitranscriptome, such as N6-Methyladenosine (m6A) of mRNA, is an abundant post-transcriptional mRNA modification and has been recently implicated to play roles in several cancers, whereas the significance of m6A modifications is virtually unknown in ESCC. Analysis of tissue microarray of the tumors in 177 ESCC patients revealed that higher expression of m6A demethylase ALKBH5 correlated with poor prognosis and that ALKBH5 was an independent prognostic factor of the survival of patients. There was no correlation between the other demethylase FTO and prognosis. siRNA knockdown of ALKBH5 but not FTO significantly suppressed proliferation and migration of human ESCC cells. ALKBH5 knockdown delayed progression of cell cycle and accumulated the cells to G0/G1 phase. Mechanistically, expression of CDKN1A (p21) was significantly upregulated in ALKBH5-depleted cells, and m6A modification and stability of CDKN1A mRNA were increased by ALKBH5 knockdown. Furthermore, depletion of ALKBH5 substantially suppressed tumor growth of ESCC cells subcutaneously transplanted in BALB/c nude mice. Collectively, we identify ALKBH5 as the first m6A demethylase that accelerates cell-cycle progression and promotes cell proliferation of ESCC cells, which is associated with poor prognosis of ESCC patients.

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Research Achievements 【 display / non-display 】

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Presentations 【 display / non-display 】

  • リンパ節転移陽性胸部食道扁平上皮癌に対する術前化学放射線療法+手術 (PET-Nを用いた予後予測)

    長岐　雄志、本山　悟、佐藤　雄亮、脇田　晃行、藤田　啓、煙山　紘平、佐々木　吉寛、今井　一博、南谷　佳弘

    第76回日本消化器外科学会定期学術集会  2021.07  -  2021.07 

  • 術前CRT+手術を施行した食道扁平上皮癌のCRT Grade別の治療成績の検討

    長岐　雄志、本山　悟、佐藤　雄亮、脇田　晃行、川北　雄太、南谷　佳弘

    第74回日本食道学会学術集会  2020.12  -  2020.12 

  • m6A demethylase ALKBH5 promotes proliferation of esophageal squamous cell carcinoma associated with poor prognosis.

    長岐　雄志、久場　敬司

    第79回日本癌学会学術集会  2020.10  -  2020.10 

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