Affiliation |
Graduate School of Medicine Doctorial Course in Medicine Oncoregulatory Medicine Department of Thoracis Surgery |
Date of Birth |
1987 |
NAGAKI Yushi
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Research Interests 【 display / non-display 】
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リンパ節転移
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転移
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癌
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消化器癌
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食道癌
Graduating School 【 display / non-display 】
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2006.04-2012.03
Akita University Faculty of Medicine Graduated
Graduate School 【 display / non-display 】
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2014.04-2020.09
Akita University Graduate School, Division of Medicine Doctor's Course Completed
Campus Career 【 display / non-display 】
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2022.07-Now
Akita University Graduate School of Medicine Doctorial Course in Medicine Oncoregulatory Medicine Department of Thoracis Surgery Assistant Professor
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2017.04-2021.03
Akita University Graduate School of Medicine Doctorial Course in Medicine Oncoregulatory Medicine Department of Thoracis Surgery Graduate Student
Thesis for a degree 【 display / non-display 】
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m6A demethylase ALKBH5 promotes proliferation of esophageal squamous cell carcinoma associated with poor prognosis.
Yushi Nagaki, Satoru Motoyama, Tomokazu Yamaguchi, Midori Hoshizaki, Yusuke Sato, Teruki Sato, Yukio Koizumi, Akiyuki Wakita, Yuta Kawakita, Kazuhiro Imai, Hiroshi Nanjo, Hiroyuki Watanabe, Yumiko Imai, Yoshihiro Minamiya, Keiji Kuba
Genes to cells 2020.09 [Refereed]
Single author
Esophageal squamous cell carcinoma (ESCC) is one of the most fatal types of malignant tumors worldwide. Epitranscriptome, such as N6-Methyladenosine (m6A) of mRNA, is an abundant post-transcriptional mRNA modification and has been recently implicated to play roles in several cancers, whereas the significance of m6A modifications is virtually unknown in ESCC. Analysis of tissue microarray of the tumors in 177 ESCC patients revealed that higher expression of m6A demethylase ALKBH5 correlated with poor prognosis and that ALKBH5 was an independent prognostic factor of the survival of patients. There was no correlation between the other demethylase FTO and prognosis. siRNA knockdown of ALKBH5 but not FTO significantly suppressed proliferation and migration of human ESCC cells. ALKBH5 knockdown delayed progression of cell cycle and accumulated the cells to G0/G1 phase. Mechanistically, expression of CDKN1A (p21) was significantly upregulated in ALKBH5-depleted cells, and m6A modification and stability of CDKN1A mRNA were increased by ALKBH5 knockdown. Furthermore, depletion of ALKBH5 substantially suppressed tumor growth of ESCC cells subcutaneously transplanted in BALB/c nude mice. Collectively, we identify ALKBH5 as the first m6A demethylase that accelerates cell-cycle progression and promotes cell proliferation of ESCC cells, which is associated with poor prognosis of ESCC patients.
Research Achievements 【 display / non-display 】
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Motoyama S.
Annals of Surgery ( Annals of Surgery ) 276 ( 1 ) E16 - E23 2022.07
Research paper (journal)
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Nagaki Y.
Annals of Surgical Oncology ( Annals of Surgical Oncology ) 29 ( 2 ) 1336 - 1346 2022.02
Research paper (journal) Domestic Co-author
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Nagaki Y.
BMC Cancer ( BMC Cancer ) 21 ( 1 ) 2021.12 [Refereed]
Research paper (journal) Domestic Co-author
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Nagaki Y.
World Journal of Surgical Oncology ( World Journal of Surgical Oncology ) 19 ( 1 ) 2021.12
Research paper (journal) Domestic Co-author
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Motoyama S.
Cancer Science ( Cancer Science ) 112 ( 10 ) 4281 - 4291 2021.10
Research paper (journal)
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Important points before starting robot-assisted esophagectomy
74 ( 3 ) 294 - 297 2019.03
◆Original paper【 display / non-display 】
◆Other【 display / non-display 】
Presentations 【 display / non-display 】
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リンパ節転移陽性胸部食道扁平上皮癌に対する術前化学放射線療法+手術 (PET-Nを用いた予後予測)
長岐 雄志、本山 悟、佐藤 雄亮、脇田 晃行、藤田 啓、煙山 紘平、佐々木 吉寛、今井 一博、南谷 佳弘
第76回日本消化器外科学会定期学術集会 2021.07 - 2021.07
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術前CRT+手術を施行した食道扁平上皮癌のCRT Grade別の治療成績の検討
長岐 雄志、本山 悟、佐藤 雄亮、脇田 晃行、川北 雄太、南谷 佳弘
第74回日本食道学会学術集会 2020.12 - 2020.12
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m6A demethylase ALKBH5 promotes proliferation of esophageal squamous cell carcinoma associated with poor prognosis.
長岐 雄志、久場 敬司
第79回日本癌学会学術集会 2020.10 - 2020.10