研究等業績 - 原著論文 - 関　信輔

Cryopreservation of rat embryos at all developmental stages by small-volume vitrification procedure and rapid warming in cryotubes

Seki S.

Scientific Reports ( Scientific Reports )  13 ( 1 ) 20903 - 20903   2023年12月

研究論文（学術雑誌）  

Intracellular ice formation during cryopreservation is lethal to the cell, including during warming. Here, we examined the effect of sample volume and warming rate on the cryopreservation success of 1-cell rat embryos based on successful development into blastocysts in vitro and to term in vivo following embryo transfer. Embryos were equilibrated in 5% propylene glycol solution for 10 min, held for 40 s at 0 °C in cryopreservation solution (5%PG + PEPeS), and cooled by immersion in liquid nitrogen. When 1-cell embryos were cryopreserved in a volume of 30-100 μL at a cooling rate of 5830-7160 °C/min and warmed at 35,480-49,400 °C/min by adding 1 mL of 0.3 M sucrose solution at 50 °C, 17.3-28.8% developed into blastocysts, compared with 57.0% of untreated embryos. However, when 1-cell embryos were cryopreserved in a smaller volume of 15 μl at 7950 °C/min and warmed at 68,850 °C/min, 58.8 ± 10.6% developed into blastocysts and 50.0 ± 7.4% developed to term, comparable to that of non-treated embryos (57.0 ± 5.4% and 51.4 ± 3.1%, respectively). Cryopreserved embryos at other developmental stages also showed high in vitro culture potential similar to that of the control. Using a conventional cryotube and a small-volume vitrification procedure with rapid warming, we achieved high levels of subsequent rat embryonic development at all developmental stages.

DOI PubMed

TIGIT mediates activation-induced cell death of ILC2sduring chronic airway allergy

Yamada T.

Journal of Experimental Medicine ( Journal of Experimental Medicine )  220 ( 7 )   2023年07月

研究論文（学術雑誌）  

While group-2 innate lymphoid cells (ILC2s) are highly proliferative in allergic inflammation, the removal of overactivated ILC2s in allergic diseases has not been investigated. We previously showed that chronic airway allergy induces "exhausted-like" dysfunctional ILC2s expressing T cell immunoreceptor with Ig and ITIM domains (TIGIT). However, the physiological relevance of these cells in chronic allergy remains elusive. To precisely identify and monitor TIGIT+ ILC2s, we generated TIGIT lineage tracer mice. Chronic allergy stably induced TIGIT+ ILC2s, which were highly activated, apoptotic, and were quickly removed from sites of chronic allergy. Transcripts from coding genes were globally suppressed in the cells, possibly due to reduced chromatin accessibility. Cell death in TIGIT+ ILC2s was enhanced by interactions with CD155 expressed on macrophages, whereas genetic ablation of Tigit or blockade by anti-TIGIT antagonistic antibodies promoted ILC2 survival, thereby deteriorating chronic allergic inflammation. Our work demonstrates that TIGIT shifts the fate of ILC2s toward activation-induced cell death, which could present a new therapeutic target for chronic allergies.

DOI PubMed

Streamlined and quantitative detection of chimerism using digital PCR

Sachy FP, Nishimura T, Seki S, Wilkinson AC, Higuchi M, Hsu I, Zhang J, Bhadury J, Nakauchi, H

Scientific Reports ( Scientific Reports )  12 ( 1 )   2022年12月  [査読有り]

研究論文（学術雑誌）   国際共著

DOI

Small-volume vitrification and rapid warming yield high survivals of one-cell rat embryos in cryotubes

Fukuda Y, Higashiya M, Obata T, Basaki K, Yano M, Matsumura K, Ono K, Ohba T, Okamoto Y, Nishijima K, Seki S

Biology of Reproduction ( Biology of Reproduction )  105 ( 1 ) 258 - 266   2021年07月  [査読有り]

研究論文（学術雑誌）   国内共著

DOI PubMed

Characteristic features of newly established specific pathogen-free albino large rabbit (JW-AKT): Comparison with Japanese white and new Zealand white rabbits

Matsuda Y, Shibata Y, Basaki K, Fukuda Y, Takaki N, Maeda T, Hirao M, Yano M, Higashiya M, Obata T, Seki S, Nishijima K

Journal of Veterinary Medical Science ( Journal of Veterinary Medical Science )  81 ( 5 ) 739 - 743   2019年05月  [査読有り]

研究論文（学術雑誌）   国内共著

DOI PubMed CiNii Research

潜性致死遺伝子を標的としたゲノム編集法について

場﨑 恵太, 福田 康義, 矢野 愛美, 東谷美沙子, 小畑 孝弘, 関 信輔, 西島 和俊

九州実験動物雑誌   35   45 - 51   2019年  [査読有り]

研究論文（学術雑誌）  

Vitrification of one-cell mouse embryos in cryotubes

Seki S, Basaki K, Komatsu Y, Fukuda Y, Yano M, Matsuo Y, Obata T, Matsuda Y, Nishijima K

Cryobiology ( Cryobiology )  81   132 - 137   2018年04月  [査読有り]

研究論文（学術雑誌）  

DOI PubMed

The CCR4-NOT deadenylase complex controls atg7-dependent cell death and heart function

Yamaguchi T, Suzuki T, Sato T, Takahashi A, Watanabe H, Kadowaki A, Natsui M, Inagaki H,Arakawa S, Nakaoka S, Koizumi Y, Seki S, Adachi S, Fukao A, Fujiwara T, Natsume T, Kimura A, Komatsu M, Shimizu S, Ito K, Suzuki Y, Penninger JM, Yamamoto T, Imai Y, Kuba K

Science Signaling ( Science Signaling )  11 ( 516 )   2018年02月  [査読有り]

研究論文（学術雑誌）   国内共著

DOI PubMed

アルビノ大型ウサギSPFコロニーの樹立

松田幸久, 柴田淑子, 場﨑恵太, 福田康義, 矢野愛美, 東谷美沙子, 小畑孝弘, 関信輔, 西島和俊

九州実験動物雑誌   34   23 - 27   2018年  [査読有り]

研究論文（学術雑誌）   国内共著

Production of the medaka derived from vitrified whole testes by germ cell transplantation

Seki S, Kusano K, Lee S, Iwasaki Y, Yagisawa M, Ishida M, Sasado T, Naruse K, Yoshizaki G

Scientific Reports ( Scientific Reports )  7   43185 - 43185   2017年03月  [査読有り]

研究論文（学術雑誌）   国内共著

DOI PubMed

Production of viable trout offspring derived from frozen whole fish

Lee S, Seki S, Katayama N, Yoshizaki G

Scientific Reports   2   16045   2015年11月  [査読有り]

研究論文（学術雑誌）   国内共著

Extreme rapid warming yields high functional survivals of vitrified 8-cell mouse embryos even when suspended in a half-strength vitrification solution and cooled at moderate rates to -196oC

Seki S, Jin B, Mazur P

Cryobiology   68   71 - 78   2014年04月  [査読有り]

研究論文（学術雑誌）   国内共著

A trial to cryopreserve Immature medaka (Oryzias latipes) oocytes after enhancing their permeability by exogenous expression of aquaporin 3

Valdez DMJr, Tsuchiya R, Seki S, Saida N, Niimi S, Koshimoto C, Matsukawa K, Kasai M, Edashige K

Journal of Reproduction and Development   59   205 - 213   2013年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Ultra-rapid warming yields high survival of mouse oocytes cooled to -196 oC in dilutions of a standard vitrification solution

Seki S, Mazur P

PLoS ONE   7   e36058   2012年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Survival of mouse oocytes after being cooled in a vitrification solution to -196oC at 95o to 70,000oC/min and warmed at 610o to 118,000oC/min: A new paradigm for cryopreservation by vitrification

Mazur P, Seki S, Pinn IL, Kleinhans FW, Edashige K

Cryobiology   62   1 - 7   2011年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Cryobiological properties of immature zebrafish oocytes assessed by their ability to fertilize and develop into hatching embryos

Seki S, Kouya T, Tsuchiya R, Valdez DMJr, Jin B, Koshimoto C, Kasai M, Edashige K

Cryobiology   62   8 - 14   2011年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Stability of mouse oocytes at -80 oC: The role of the recrystallization of intracellular

Seki S, Mazur P

Reproduction   141   407 - 415   2011年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Developmental ability of vitrified mouse oocyte expressing water channels

Yamaji Y, Seki S, Matsukawa K, Koshimoto C, Kasai M, Edashige K

Journal of Reproduction and Development   57   403 - 408   2011年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Pathway for the movement of water and cryoprotectants in bovine oocytes and embryos

Jin B, Kawai Y, Hara T, Takeda S, Seki S, Nakata Y, Matsukawa K, Koshimoto C, Kasai M, Edashige K

Biology of Reproduction   85   834 - 847   2011年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Effect of the expression of aquaporins 1 and 3 in mouse oocytes and embryos on the nucleation temperature for intracellular ice formation

Seki S, Edashige K, Wada S, Mazur P

Reproduction   142   505 - 515   2011年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Equilibrium vitrification of mouse embryos

Jin B, Mochida K, Ogura A, Hotta E, Kobayashi Y, Ito K, Egawa G, Seki S, Honda H, Edashige K, Kasai M

Biology of Reproduction   82   444 - 450   2010年04月  [査読有り]

研究論文（学術雑誌）   国内共著

The temperature and type of intracellular ice formation in preimplantation mouse embryos as a function of the developmental stage

Seki S, Mazur P

Biology of Reproduction   82   1198 - 1205   2010年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Comparison between the temperatures of intracellular ice formation in fresh mouse oocytes and embryos and those previously subjected to a vitrification procedure

Seki S, Mazur P

Cryobiology   61   155 - 157   2010年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Simple, inexpensive attainment and measurement of very high cooling and warming rates

Kleinhans FW, Seki S, Mazur P

Cryobiology   61   231 - 233   2010年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Intracellular ice formation in cells of the yeast Saccharomyces cerevisiae as a function of cooling rate: predictions from modeling vs. experimental observations by differential scanning calorimetry

Seki S, Kleinhans FW, Mazur P

Cryobiology   58   157 - 165   2009年04月  [査読有り]

研究論文（学術雑誌）   国内共著

The dominance of warming rate over cooling rate in the survival of mouse oocytes subjected to a vitrification procedure

Seki S, Mazur P

Cryobiology   59   75 - 82   2009年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Development of a reliable in vitro maturation system for zebrafish oocytes

Seki S, Kouya T, Tsuchiya R, Valdez DMJr, Jin B, Hara T, Saida N, Kasai M, Edashige K

Reproduction   135   285 - 292   2008年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Kinetics and activation energy of recrystallization of intracellular ice in mouse oocytes subjected to interrupted rapid cooling

Seki S, Mazur P

Cryobiology   56   171 - 180   2008年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Formation of extracellular and intracellular ice during warming of vitrified mouse morulae and its effect on embryo survival

Jin B, Kusanagi K, Ueda M, Seki S, Valdez DMJr, Edashige K, Kasai M

Cryobiology   56   233 - 240   2008年04月  [査読有り]

研究論文（学術雑誌）   国内共著

The mechanism by which mouse spermatozoa are injured during freezing

Jin B, Yamasaki C, Yamada N, Seki S, Valdez DMJr, Kasai M, Edashige K

Journal of reproduction and development   54   265 - 269   2008年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Effect of warming rate on the survival of vitrified mouse oocytes and on the recrystallization of intracellular ice

Seki S, Mazur P

Biology of Reproduction   79   727 - 737   2008年04月  [査読有り]

研究論文（学術雑誌）   国内共著

The permeability to water and cryoprotectants of immature and mature oocytes in the zebrafish (Danio rerio)

Seki S, Kouya T, Valdez DMJr, Jin B, Hara T, Saida N, Kasai M, Edashige K

Cryobiology   54   121 - 124   2007年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Exogenous expression of rat aquaporin-3 enhances permeability to water and cryoprotectants of immature oocytes in the zebrafish (Danio rerio)

Seki S, Kouya T, Hara T, Valdez DMJr, Jin B, Kasai M, Edashige K

Journal of Reproduction and Development   53   597 - 604   2007年04月  [査読有り]

研究論文（学術雑誌）   国内共著

The role of aquaporin 3 in the movement of water and cryoprotectants in mouse morulae

Edashige K, Ohta S, Tanaka M, Kuwano T, Valdez DMJr, Hara T, Jin B, Takahashi S, Seki S, Koshimoto C, Kasai M

Biology of Reproduction   77   365 - 375   2007年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Extra- and intra-cellular ice formation in Stage I and II Xenopus laevis oocytes

Guenther JF, Seki S, Kleinhans FW, Edashige K, Roberts DM, Mazur P

Cryobiology   52   401 - 416   2006年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Japanese flounder (Paralichthys olivaceus) embryos are difficult to cryopreserve by vitrification

Edashige K, Valdez DMJr, Hara T, Saida N, Seki S, Kasai M

Cryobiology   53   96 - 106   2006年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Expression of aquaporin-3 improves the permeability to water and cryoprotectants of immature oocytes in the medaka (Oryzias latipes)

Valdez DMJr, Hara T, Miyamoto A, Seki S, Jin B, Kasai M, Edashige K

Cryobiology   53   160 - 168   2006年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Cryoprotectant permeability of aquaporin-3 expressed in Xenopus oocytes

Yamaji Y, Valdez DMJr, Seki S, Yazawa K, Urakawa C, Jin B, Kasai M, Kleinhans FW, Edashige K

Cryobiology   53   258 - 267   2006年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Water- and cryoprotectant-permeability of mature and immature oocytes in the medaka (Oryzias latipes)

Valdez DMJr, Miyamoto A, Hara T, Seki S, Kasai M, Edashige K

Cryobiology   50   93 - 102   2005年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Extra- and intracellular ice formation in mouse oocytes

Mazur P, Seki S, Pinn IL, Kleinhans FW, Edashige K

Cryobiology   51   29 - 53   2005年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Effects of hold time after extracellular ice formation on intracellular freezing of mouse oocytes

Mazur P, Pinn IL, Seki S, Kleinhans FW, Edashige K

Cryobiology   51   235 - 239   2005年04月  [査読有り]

研究論文（学術雑誌）   国内共著