研究等業績 - 原著論文 - 関　信輔

Cryopreservation of rat embryos at all developmental stages by small-volume vitrification procedure and rapid warming in cryotubes

Seki S.

Scientific Reports ( Scientific Reports )  13 ( 1 ) 20903 - 20903   2023年12月

研究論文（学術雑誌）  

Intracellular ice formation during cryopreservation is lethal to the cell, including during warming. Here, we examined the effect of sample volume and warming rate on the cryopreservation success of 1-cell rat embryos based on successful development into blastocysts in vitro and to term in vivo following embryo transfer. Embryos were equilibrated in 5% propylene glycol solution for 10 min, held for 40 s at 0 °C in cryopreservation solution (5%PG + PEPeS), and cooled by immersion in liquid nitrogen. When 1-cell embryos were cryopreserved in a volume of 30-100 μL at a cooling rate of 5830-7160 °C/min and warmed at 35,480-49,400 °C/min by adding 1 mL of 0.3 M sucrose solution at 50 °C, 17.3-28.8% developed into blastocysts, compared with 57.0% of untreated embryos. However, when 1-cell embryos were cryopreserved in a smaller volume of 15 μl at 7950 °C/min and warmed at 68,850 °C/min, 58.8 ± 10.6% developed into blastocysts and 50.0 ± 7.4% developed to term, comparable to that of non-treated embryos (57.0 ± 5.4% and 51.4 ± 3.1%, respectively). Cryopreserved embryos at other developmental stages also showed high in vitro culture potential similar to that of the control. Using a conventional cryotube and a small-volume vitrification procedure with rapid warming, we achieved high levels of subsequent rat embryonic development at all developmental stages.

DOI PubMed

TIGIT mediates activation-induced cell death of ILC2sduring chronic airway allergy

Yamada T.

Journal of Experimental Medicine ( Journal of Experimental Medicine )  220 ( 7 )   2023年07月

研究論文（学術雑誌）  

While group-2 innate lymphoid cells (ILC2s) are highly proliferative in allergic inflammation, the removal of overactivated ILC2s in allergic diseases has not been investigated. We previously showed that chronic airway allergy induces "exhausted-like" dysfunctional ILC2s expressing T cell immunoreceptor with Ig and ITIM domains (TIGIT). However, the physiological relevance of these cells in chronic allergy remains elusive. To precisely identify and monitor TIGIT+ ILC2s, we generated TIGIT lineage tracer mice. Chronic allergy stably induced TIGIT+ ILC2s, which were highly activated, apoptotic, and were quickly removed from sites of chronic allergy. Transcripts from coding genes were globally suppressed in the cells, possibly due to reduced chromatin accessibility. Cell death in TIGIT+ ILC2s was enhanced by interactions with CD155 expressed on macrophages, whereas genetic ablation of Tigit or blockade by anti-TIGIT antagonistic antibodies promoted ILC2 survival, thereby deteriorating chronic allergic inflammation. Our work demonstrates that TIGIT shifts the fate of ILC2s toward activation-induced cell death, which could present a new therapeutic target for chronic allergies.

DOI PubMed

Streamlined and quantitative detection of chimerism using digital PCR

Sachy FP, Nishimura T, Seki S, Wilkinson AC, Higuchi M, Hsu I, Zhang J, Bhadury J, Nakauchi, H

Scientific Reports ( Scientific Reports )  12 ( 1 )   2022年12月  [査読有り]

研究論文（学術雑誌）   国際共著

DOI

Small-volume vitrification and rapid warming yield high survivals of one-cell rat embryos in cryotubes

Fukuda Y, Higashiya M, Obata T, Basaki K, Yano M, Matsumura K, Ono K, Ohba T, Okamoto Y, Nishijima K, Seki S

Biology of Reproduction ( Biology of Reproduction )  105 ( 1 ) 258 - 266   2021年07月  [査読有り]

研究論文（学術雑誌）   国内共著

DOI PubMed

Characteristic features of newly established specific pathogen-free albino large rabbit (JW-AKT): Comparison with Japanese white and new Zealand white rabbits

Matsuda Y, Shibata Y, Basaki K, Fukuda Y, Takaki N, Maeda T, Hirao M, Yano M, Higashiya M, Obata T, Seki S, Nishijima K

Journal of Veterinary Medical Science ( Journal of Veterinary Medical Science )  81 ( 5 ) 739 - 743   2019年05月  [査読有り]

研究論文（学術雑誌）   国内共著

DOI PubMed CiNii Research

潜性致死遺伝子を標的としたゲノム編集法について

場﨑 恵太, 福田 康義, 矢野 愛美, 東谷美沙子, 小畑 孝弘, 関 信輔, 西島 和俊

九州実験動物雑誌   35   45 - 51   2019年  [査読有り]

研究論文（学術雑誌）  

Vitrification of one-cell mouse embryos in cryotubes

Seki S, Basaki K, Komatsu Y, Fukuda Y, Yano M, Matsuo Y, Obata T, Matsuda Y, Nishijima K

Cryobiology ( Cryobiology )  81   132 - 137   2018年04月  [査読有り]

研究論文（学術雑誌）  

DOI PubMed

The CCR4-NOT deadenylase complex controls atg7-dependent cell death and heart function

Yamaguchi T, Suzuki T, Sato T, Takahashi A, Watanabe H, Kadowaki A, Natsui M, Inagaki H,Arakawa S, Nakaoka S, Koizumi Y, Seki S, Adachi S, Fukao A, Fujiwara T, Natsume T, Kimura A, Komatsu M, Shimizu S, Ito K, Suzuki Y, Penninger JM, Yamamoto T, Imai Y, Kuba K

Science Signaling ( Science Signaling )  11 ( 516 )   2018年02月  [査読有り]

研究論文（学術雑誌）   国内共著

DOI PubMed

アルビノ大型ウサギSPFコロニーの樹立

松田幸久, 柴田淑子, 場﨑恵太, 福田康義, 矢野愛美, 東谷美沙子, 小畑孝弘, 関信輔, 西島和俊

九州実験動物雑誌   34   23 - 27   2018年  [査読有り]

研究論文（学術雑誌）   国内共著

Production of the medaka derived from vitrified whole testes by germ cell transplantation

Seki S, Kusano K, Lee S, Iwasaki Y, Yagisawa M, Ishida M, Sasado T, Naruse K, Yoshizaki G

Scientific Reports ( Scientific Reports )  7   43185 - 43185   2017年03月  [査読有り]

研究論文（学術雑誌）   国内共著

DOI PubMed

Production of viable trout offspring derived from frozen whole fish

Lee S, Seki S, Katayama N, Yoshizaki G

Scientific Reports   2   16045   2015年11月  [査読有り]

研究論文（学術雑誌）   国内共著

Extreme rapid warming yields high functional survivals of vitrified 8-cell mouse embryos even when suspended in a half-strength vitrification solution and cooled at moderate rates to -196oC

Seki S, Jin B, Mazur P

Cryobiology   68   71 - 78   2014年04月  [査読有り]

研究論文（学術雑誌）   国内共著

A trial to cryopreserve Immature medaka (Oryzias latipes) oocytes after enhancing their permeability by exogenous expression of aquaporin 3

Valdez DMJr, Tsuchiya R, Seki S, Saida N, Niimi S, Koshimoto C, Matsukawa K, Kasai M, Edashige K

Journal of Reproduction and Development   59   205 - 213   2013年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Ultra-rapid warming yields high survival of mouse oocytes cooled to -196 oC in dilutions of a standard vitrification solution

Seki S, Mazur P

PLoS ONE   7   e36058   2012年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Survival of mouse oocytes after being cooled in a vitrification solution to -196oC at 95o to 70,000oC/min and warmed at 610o to 118,000oC/min: A new paradigm for cryopreservation by vitrification

Mazur P, Seki S, Pinn IL, Kleinhans FW, Edashige K

Cryobiology   62   1 - 7   2011年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Cryobiological properties of immature zebrafish oocytes assessed by their ability to fertilize and develop into hatching embryos

Seki S, Kouya T, Tsuchiya R, Valdez DMJr, Jin B, Koshimoto C, Kasai M, Edashige K

Cryobiology   62   8 - 14   2011年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Stability of mouse oocytes at -80 oC: The role of the recrystallization of intracellular

Seki S, Mazur P

Reproduction   141   407 - 415   2011年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Developmental ability of vitrified mouse oocyte expressing water channels

Yamaji Y, Seki S, Matsukawa K, Koshimoto C, Kasai M, Edashige K

Journal of Reproduction and Development   57   403 - 408   2011年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Pathway for the movement of water and cryoprotectants in bovine oocytes and embryos

Jin B, Kawai Y, Hara T, Takeda S, Seki S, Nakata Y, Matsukawa K, Koshimoto C, Kasai M, Edashige K

Biology of Reproduction   85   834 - 847   2011年04月  [査読有り]

研究論文（学術雑誌）   国内共著

Effect of the expression of aquaporins 1 and 3 in mouse oocytes and embryos on the nucleation temperature for intracellular ice formation

Seki S, Edashige K, Wada S, Mazur P

Reproduction   142   505 - 515   2011年04月  [査読有り]

研究論文（学術雑誌）   国内共著