FUJIWARA Kenshu

写真a

Affiliation

Graduate School of Engineering Science  Department of Life Science  Life Science Course 

Date of Birth

1963

Laboratory Address

1-1 Tegata Gakuen-machi, Akita 010-8502, Japan

Laboratory Phone number

+81-18-889-2440

Laboratory Fax number

+81-18-889-2440

Homepage URL

http://www.gipc.akita-u.ac.jp/~synbioorg/index.html

Mail Address

E-mail address

Research Fields, Keywords

Organic Chemistry, Synthetic Chemistry, Natural Products, Chemistry of Bio-molecules

Graduating School 【 display / non-display

  • 1982.04
    -
    1986.03

    Tohoku University   Faculty of Science   Graduated

Graduate School 【 display / non-display

  • 1988.04
    -
    1991.03

    Tohoku University  Graduate School, Division of Natural Science  Doctor's Course  Completed

  • 1986.04
    -
    1986.03

    Tohoku University  Graduate School, Division of Natural Science  Master's Course  Completed

Campus Career 【 display / non-display

  • 2016.04
    -
    Now

    Akita University   Graduate School of Engineering Science   Department of Life Science   Professor  

  • 2015.10
    -
    2016.03

    Akita University   Graduate School of Engineering and Resource Science   Department of Life Science   Professor  

External Career 【 display / non-display

  • 2009.10
    -
    2010.03

    Niigata University  

Research Field (grants-in-aid-for-scientific-research classification) 【 display / non-display

  • Biomolecular chemistry

  • Bioorganic chemistry

  • Synthetic chemistry

  • Organic chemistry

 

Published Papers 【 display / non-display

  • Total synthesis of selaginellin A

    K. Fujiwara, T. Itagaki, T. Tokiwano

    Tetrahedron Letters ( Tetrahedron Letters )  79   153295   2021.08  [Refereed]

    Domestic Co-author

    The total synthesis of selaginellin A, isolated from Selaginella tamariscina, was achieved through an eight-step process including a Diels-Alder reaction, formation of a quinone methide moiety and dehydrogenative aromatization. The NMR spectra collected for different samples of synthetic selaginellin A initially showed irregular peak shapes and variable chemical shifts for the signals of the phenol-substituted quinone methide moiety under neutral conditions. After detailed NMR analysis, it was found that the addition of a trace amount of trifluoroacetic acid accelerated the tautomerism of the phenol-substituted quinone methide moiety and improved the reproducibility of the chemical shifts of synthetic selaginellin A.

    DOI

  • 4-(2,5-Dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide improves monoclonal antibody production in a Chinese hamster ovary cell culture

    Y. Aki, Y. Katsumata, H. Kakihara, K. Nonaka, K. Fujiwara

    PLoS ONE ( PLoS ONE )  16 ( 4 April 2021 ) e0250416   2021.04  [Refereed]

    Domestic Co-author

    There is a continuous demand to improve monoclonal antibody production for medication supply and medical cost reduction. For over 20 years, recombinant Chinese hamster ovary cells have been used as a host in monoclonal antibody production due to robustness, high productivity and ability to produce proteins with ideal glycans. Chemical compounds, such as dimethyl sulfoxide, lithium chloride, and butyric acid, have been shown to improve monoclonal antibody production in mammalian cell cultures. In this study, we aimed to discover new chemical compounds that can improve cell-specific antibody production in recombinant Chinese hamster ovary cells. Out of the 23,227 chemicals screened in this study, 4-(2,5-dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide was found to increase monoclonal antibody production. The compound suppressed cell growth and increased both cell-specific glucose uptake rate and the amount of intracellular adenosine triphosphate during monoclonal antibody production. In addition, the compound also suppressed the galactosylation on a monoclonal antibody, which is a critical quality attribute of therapeutic monoclonal antibodies. Therefore, the compound might also be used to control the level of the galactosylation for the N-linked glycans. Further, the structure-activity relationship study revealed that 2,5-dimethylpyrrole was the most effective partial structure of 4-(2,5-dimethyl-1H-pyrrol-1-yl)-N-(2,5-dioxopyrrolidin-1-yl) benzamide on monoclonal antibody production. Further structural optimization of 2,5-dimethylpyrrole derivatives could lead to improved production and quality control of monoclonal antibodies.

    DOI PubMed

  • Total synthesis of selaginellin S

    Kenshu Fujiwara, Takaya Itagaki, Yoshihiko Kondo, Uichi Akiba, Tetsuo Tokiwano

    Tetrahedron Letters ( Tetrahedron Letters )  61 ( 25 ) 152031   2020.06  [Refereed]

    Domestic Co-author

    DOI

  • An Approach to a 2-Hydroxy-3-phenyldibenzofuran Skeleton Based on Rh(PPh3)3Cl-Catalyzed [2+2+2] Cycloaddition Between a 1-Ethynyl-2-(Ethynyloxy)benzene and an (Alkoxyethynyl)benzene

    Daisuke Sato, Kenshu Fujiwara, Yoshihiko Kondo, Uichi Akiba, Tetsuo Tokiwano

    HETEROCYCLES   101 ( 2 ) 417 - 422   2020.01  [Refereed]

    Domestic Co-author

  • Molecular Wires with Controllable π‐Delocalization Incorporating Redox‐Triggered π‐Conjugated Switching Units.

    Wataru Nojo, Hitomi Tamaoki, Yusuke Ishigaki, Ryo Katoono, Kenshu Fujiwara, Takanori Fukushima, Takanori Suzuki

    ChemPlusChem   84 ( 6 ) 634 - 642   2019.07  [Refereed]

    Domestic Co-author

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Grant-in-Aid for Scientific Research 【 display / non-display

  • Grant-in-Aid for Scientific Research(C)

    Project Year: 2018.04  -  2021.03 

  • Grant-in-Aid for Scientific Research(B)

    Project Year: 2015.04  -  2019.03 

  • Grant-in-Aid for Scientific Research(C)

    Project Year: 2015.04  -  2018.03 

  • Grant-in-Aid for Scientific Research(C)

    Project Year: 2012.04  -  2015.03 

  • Grant-in-Aid for Challenging Research (Pioneering)/(Exploratory)

    Project Year: 2011.04  -  2012.03 

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Presentations 【 display / non-display

  • Total Synthesis of Pectenotoxin-2

    Kenshu Fujiwara

    2011 Asian Core Program Lectureship Award Lecture  (Hsinchu, Taiwan)  2012.11  -  2012.11  Asian Core Program