研究等業績 - その他 - 鈴木 真輔
-
椎名 和弘, 山崎 一春, 本田 耕平, 山田 武千代, 宮部 結, 登米 慧, 齋藤 秀和, 辻 正博, 小泉 洸, 川嵜 洋平, 佐藤 輝幸, 鈴木 真輔
耳鼻咽喉科展望 ( 耳鼻咽喉科展望会 ) 62 ( 1 ) s18 - s19 2019年
-
登米 慧, 宮部 結, 椎名 和弘, 飯川 延子, 齋藤 秀和, 小泉 洸, 川嵜 洋平, 鈴木 真輔, 山田 武千代
耳鼻咽喉科展望 ( 耳鼻咽喉科展望会 ) 62 ( 1 ) s44 - s45 2019年
-
川嵜 洋平, 辻 正博, 鈴木 真輔, 山田 武千代
頭頸部外科 ( 特定非営利活動法人 日本頭頸部外科学会 ) 27 ( 3 ) 269 - 275 2018年
内視鏡的咽喉頭手術（ELPS）は早期癌に対して非常に有効な治療法であり，手術時間も短く不要な放射線化学療法を避ける事が可能となった。一方で，ELPSが普及する前は早期癌であっても放射線化学療法に頼らざるを得なかった。内視鏡の画像精度が上がっている為，以前に放射線化学療法で加療した患者の中下咽頭癌を早期の段階で見つける事が可能となっている。しかし，こうした患者に対してELPSを施行する時，手術操作が困難であったり，腫瘍が予想外に進展していたり，切除範囲が明確に解らない場合がある。われわれが経験した症例を検討し，根治照射後のELPSの問題点を明らかにしていく。
-
飯川 延子, 鈴木 真輔, 本田 耕平, 近江 永豪, 佐藤 輝幸, 川嵜 洋平, 辻 正博, 山﨑 一春, 石川 和夫
耳鼻咽喉科展望 ( 耳鼻咽喉科展望会 ) 60 ( 1 ) s16 - s17 2017年
-
秋田大学における舌癌治療成績:―センチネルリンパ節生検の検討―
齋藤 秀和, 飯川 延子, 川嵜 洋平, 佐藤 輝幸, 近江 永豪, 鈴木 真輔, 本田 耕平, 石川 和夫
耳鼻咽喉科展望 ( 耳鼻咽喉科展望会 ) 60 ( 1 ) s38 - s39 2017年
-
Hepatic Stellate Cells : Unique Characteristics in Cell Biology and Phenotype
Sato Mitsuru, Suzuki Shinsuke, Senoo Haruki
Cell structure and function ( Japan Society for Cell Biology ) 28 ( 2 ) 105 - 112 2003年04月
-
Sato Mituru, Sato Takeya, Kojima Naosuke, Miura Mitsutaka, Imai Katsuyuki, Wang DaRen, Higashi Nobuyo, Suzuki Shinsuke, Senoo Haruki
Connective tissue ( 日本結合組織学会 ) 34 ( 1 ) 2002年
Hepatic stellate cells (HSCs), vitamin A-storing cells located in the perisinusoidal spaces between the sinusoidal endothelial cell lining and the parenchymal cell cord, extend long cellular processes and surround hepatic sinusoids in vivo. However, when isolated and cultured in polystyrene dishes or in type I collagen coated dishes, HSCs lost vitamin A-containing lipid droplets and altesed to myofibroblast-like phenotype with well-developed stress fibers and without cellular processes. In this study, we examined a moiphological change and a differential gene expression by histological and differential display studies in HSCs cultured using type I collagen gel as a substratum. When cultured on type I collagen gel, HSCs exhibited an in vivo morphology with long cellular processes. The results from these studies suggest that HSCs were recognizable two- or three-dimensional stmcture of extiacellular type I collagen fibrils and induced to elongate their procsesses via intracellular signaling and microtubule reorganization. The lability of the processes to cold-treatment at 4 ℃ suggested that the elongated processes of HSCs were composed of a dendrite-type of mierotubules. We demonstrated the involvement of microtubule-associated protein 2 (MAP2) in process elongation in cultured HSCs by immunofluorescence staining and immunoblofting. MAP2 mRNA levels were quantified by real-time RT-PCR, indicating no increase in MAP2 mRNA level in HSCs cultured using type I collagen gel. Therefore, the MAP2 activity appeared to be posttranscnptionally regulated in cultured HSCs. The results from differential display analysis indicated that several mRNA species including transcription factor SP1, breast cancer resistant protein (BCRP), dystonin, and KIF-associated protein 3 (KAP3) were differentially regulated by extracellular type I collagen. Dystonin and KAP3 are known to be involved in cytoskeleton organization and function. Taken together, cell surface binding to extracellular type I collagen fibrils may trigger an alteration in expression of cytoskeleton-related proteins such as MAP2, dystonin, and KAP3, in association with reorganization of microtubule for process elongation.
-
Wang Da-Ren, Sato Mitsuru, Sato Takeya, Miura Mitsutaka, Kojima Naosuke, Imai Katsuyuki, Higashi Nobuyo, Suzuki Shinsuke, Senoo Haruki
Connective tissue ( 日本結合組織学会 ) 34 ( 1 ) 2002年
Hepatic stellate cells (HSC) were found to change their morphology and function including production of matrix metalloproteinases (MMPs) in response to extracellular matrix (ECM) components used as a substratum in culture. We examined in this study the regulatory role of ECM components in expression of MMPs and tissue inhibitor of metalloproteinase (TIMP) in rat cultured HSCs. HSCs were isolated by the collagenase perfusion method, followed by centrifugation on the Percoll density gradient, and cultured in polystyrene dish, on type I collagen-coated surface, on type I collagen gel, or on Matrigel containing basement membrane components, respectively. When cultured on type I collagen gel, HSCs showed the asteroid cell shape with many elongated processes, and displayed MMP-1 activity in the culture medium, as detected by in situ zymography. Expression of MMP-1 protein and mRNA were also enhanced in HSCs cultured on type I collagen gel, as observed by immunofluorescence staining and RT-PCR analysis. Active form of MMP-2 was detected by gelatin zymography in the conditioned medium of HSCs cultured on type I collagen gel, whereas proMMP-2 but not active form of MMP-2 was detected when HSCs were cultured on polystyrene surface, on type I collagen-coated surface, or on Matrigel. Increased MMP-2 mRNA was also detected by RT-PCR in HSCs cultured on type I collagen gel, as compared to the HSCs cultured on polystyrene surface, on type I collagen-coated surface, or on Matiigel. MT1-MMP protein and mRNA expression, as examined by immunoblotting and RT-PCR analysis, were greatly enhanced in HSCs cultured on type I collagen gel, suggesting an involvement of MT1-MMP in proMMP-2 activation. TIMP-2 mRNA expression was also detected by RT-PCR analysis in HSCs cultured on type I collagen-coated surface or on type I collagen gel, although it remains unclear whether TIMP-2 is involved in proMMP-2 activation or in suppression of active form of MMP-2 in HSC culture. These results indicate that the expression of MMPs and TIMP-2 in cultured HSCs is regulated by ECM components used as substratum, suggesting an important role of HSCs in the remodelling of ECM in perisinusoidal spaces of the liver tissue.