研究等業績 - その他 - 後藤 猛
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Comparative study of the microstructure of solid rubber from Ficus carica and Hevea brasiliensis
Yokota-Imai S.
Polymers for Advanced Technologies ( Polymers for Advanced Technologies ) 32 ( 11 ) 4397 - 4405 2021年11月
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Stevens M.G.
Journal of Chemical Engineering of Japan ( Journal of Chemical Engineering of Japan ) 53 ( 9 ) 485 - 493 2020年
<p>A novel molecular imprinting technology was developed to prepare highly selective sorbents, composed of adhesive peptides and microporous silica particles, for the adsorption of target metal ions from aqueous environments. Using solid-phase peptide-synthesis, tyrosine-, lysine-, and histidine-containing peptides were tailored to contain tyrosine-, lysine- and histidine-rich regions. The tyrosine residues of the peptide were converted to 3,4-dihydroxy-L-phenylalanine (DOPA) residues to give the peptides adhesive properties mimicking the adhesion of mussel-foot proteins to wet surfaces. The resulting DOPA-peptides were incubated with target metal ions Ni(II), Cu(II), Co(II), or Zn(II), and subsequently with microporous silica particles, at pH 8.0 and 25°C. Almost all the DOPA-peptides, with the metal ions, strongly attached to the particles within 24 h and were not detached from them upon washing with an aqueous solution of pH 1–12. However, the metal ions were easily released from the particles at a pH below 7.0, implying that imprinted cavities from the target metal ions, created on the peptide-immobilized silica particles, remained after the removal of the template metal ions. The metal adsorption characteristics of the resulting particles, which were termed metal-imprinted peptide–silica particles (metal-iPSPs), were investigated. The target metal ions were adsorbed to metal-iPSPs at a pH of 8.0. The adsorption kinetics fit a pseudo-second-order model well. The adsorption isotherm was well approximated using the Langmuir model, indicating that the metal-iPSP surface was homogeneous, adsorption sites were equivalent, and the coverage formed a monolayer. The metal-iPSPs exhibited a significant and high selectivity for the imprinted target metal ions in the presence of an equimolar quantity of competitive metal ions.</p>
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Biomimetic and Bioinspired Biotechnology
Bentley W.E.
Biotechnology Journal ( Biotechnology Journal ) 13 ( 12 ) 2018年12月
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Aggregation properties as potential markers for classifying rubber particle proteins
横田 早希, 後藤 猛
Atlas of Science - 2018年
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Correlation between human renin-inhibitory activity and SPR response of saponins and unsaturated fatty acids
Gotoh, T., Kamada, T., Kikuchi, K.-I., Hori, K., Takahashi, S.
Abstract of 16th International Conference on Biopartitioning and Purification (Puerto Vallarta, Mexico). CD-ROM 1 page 2011年09月
研究論文（その他学術会議資料等） 国内共著
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Renin inhibitor in soybean
Takahashi, S., Gotoh, T., Hori, K.
Soybean - Biochemistry, Chemistry and Physiology, InTech 389 - 400 2011年01月 [招待有り]
研究論文（その他学術会議資料等） 国内共著
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Optimization of soluble human interleukin-2 production in E. Coli with coexpression of molecular chaperons in the cold induced system
Gotoh, T., Horii, I., Kikuchi, K.-I.
Abstracts of 14th International Biotechnology Symposium and Exhibition. (Rimini, Italy). CD-ROM 1 page 2010年09月
研究論文（その他学術会議資料等） 国内共著
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Expression of recombinant human (rh)-renin in Sf-9 insect cells: Application of rh-renin for inhibitor screening
Takahashi, S., Hori, K., Hiwatashi, K., Awa, H., Kikuchi, K.-I., Gotoh, T.
Abstract of 6th General Meeting of the International Proteolysis Society (Surfers Paradise, QLD Australia) CD-ROM 1 page 2009年10月
研究論文（その他学術会議資料等） 国内共著
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Characterization of prorenin processing enzyme responsible for in situ active human renin production by baculovirus-infected Sf-9 cells and inhibition of excessive processing
Gotoh, T., Awa, H., Hikage, S., Kikuchi, K.-I., Takahashi, S.
Abstract of 14th European Congress on Biotechnology (Barcelona, Spain) CD-ROM 1 page 2009年09月
研究論文（その他学術会議資料等） 国内共著