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Affiliation |
Graduate School of Engineering Science Department of Life Science Life Science Course |
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Date of Birth |
1981 |
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Laboratory Address |
Tegata Gakuen-machi 1-1, Akita, Akita, 010-8502, Japan |
YAMAGATA Nobuhiro
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Research Interests 【 display / non-display 】
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Learning and memory
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Reward
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Dopamine
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Drosophila melanogaster
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Neuroethology
Graduating School 【 display / non-display 】
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1999.04-2003.03
Tohoku University Faculty of Science Biology Graduated
Graduate School 【 display / non-display 】
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2005.04-2008.09
Tohoku University Graduate School, Division of Life Science Doctor's Degree Program Completed
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2003.04-2005.03
Tohoku University Graduate School, Division of Life Science Master's Degree Program Completed
Studying abroad experiences 【 display / non-display 】
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2021.05-2022.05
The university of Iowa Visiting Associate Professor
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2013.03-2013.09
Max-Planck-Institute of Neurobiology JSPS Postdoctral Fellowship for Research Abroad
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2010.08-2013.02
Max-Planck-Institute of Neurobiology Humboldt Postdoctral Fellow
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2009.01-2010.07
Université Toulouse III - Paul Sabatier Fyssen Postdoctral Fellow
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2005.07-2008.07
Freie Universität Berlin DAAD Exchange Scholar
Degree 【 display / non-display 】
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Tohoku University - Doctor (Life Sciences)
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Tohoku University - Master (Life Sciences)
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Tohoku University - Bachelor (Science)
Campus Career 【 display / non-display 】
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2023.04-Now
Akita University Graduate School of Engineering Science Department of Life Science Life Science Course Associate Professor
External Career 【 display / non-display 】
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2018.04-2023.03
Tohoku University Graduate School of Life Sciences Associate Professor
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2013.10-2018.03
Tohoku University Graduate School of Life Sciences Assistant Professor
Research Areas 【 display / non-display 】
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Life Science / Neuroscience-general
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Life Science / Cognitive and brain science
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Life Science / Animal physiological chemistry, physiology and behavioral biology
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Life Science / Neuroscience-general / 学習、記憶、報酬、ドーパミン
Research Achievements 【 display / non-display 】
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Shun Hiramatsu, Kokoro Saito, Shu Kondo, Hidetaka Katow, Nobuhiro Yamagata, Chun-Fang Wu, Hiromu Tanimoto
eLife ( eLife Sciences Publications, Ltd ) 2024.07 [Refereed]
Research paper (journal)
Dopamine can play opposing physiological roles depending on the receptor subtype. In the fruit fly Drosophila melanogaster, Dop1R1 and Dop2R encode the D1- and D2-like receptors, respectively, and are reported to oppositely regulate intracellular cAMP levels. Here, we profiled the expression and subcellular localization of endogenous Dop1R1 and Dop2R in specific cell types in the mushroom body circuit. For cell-type-specific visualization of endogenous proteins, we employed reconstitution of split-GFP tagged to the receptor proteins. We detected dopamine receptors at both presynaptic and postsynaptic sites in multiple cell types. Quantitative analysis revealed enrichment around the active zones, particularly for Dop2R. The presynaptic localization of Dop1R1 and Dop2R in dopamine neurons suggests dual feedback regulation as autoreceptors. Furthermore, we discovered a starvation-dependent, bidirectional modulation of the presynaptic receptor expression in the PAM and PPL1 clusters, two distinct subsets of dopamine neurons, suggesting regulation of appetitive behaviors. Our results highlight the significance of the co-expression of the two antagonizing dopamine receptors in the spatial and conditional regulation of dopamine responses in neurons.
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Aroma nudges in bugs: Sensory perception and memory in insects
Mizunami M, Yamagata N
Current Opinion in Insect Science ( Elsevier ) 61 ( 101165 ) 2024.01 [Refereed]
Research paper (journal) Domestic Co-author
Insects are truly remarkable creatures that have evolved highly advanced sensory systems to thrive in diverse environments. From their keen sense of vision to their sophisticated olfactory, gustatory, and auditory abilities, insects possess an exceptional range of sensory skills that allow them to detect, locate, and respond to the world around them. Recent research has uncovered fascinating examples of these abilities, such as the newly discovered capability of cockroaches [1] and flies [2] to detect the spatial distribution of odors. Moreover, insects like fruit flies, honeybees, and crickets exhibit extraordinary learning and memory capabilities that enable them to adapt to ever-changing environments. By studying the neural network mechanisms of learning and memory in fruit flies, we can gain invaluable insights into how these systems work at the single-neuron level 3, 4. Additionally, insect studies can provide crucial information on the adaptive significance of learning and memory [5], which is a vital area of research in ecology and evolutionary biology. In this section, we will delve into the latest breakthroughs in studying olfactory perceptions, learning, and memory in insects.
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Nutrient responding peptide hormone CCHamide-2 consolidates appetitive memory
Yamagata N, Imanishi Y, Wu H, Kondo S, Sano H, Tanimoto H.
Frontiers in Behavioral Neuroscience ( Frontiers in Behavioral Neuroscience ) 16 986064 - 986064 2022.10 [Refereed]
Research paper (journal) Domestic Co-author
CCHamide-2 (CCHa2) is a protostome excitatory peptide ortholog known for various arthropod species. In fruit flies, CCHa2 plays a crucial role in the endocrine system, allowing peripheral tissue to communicate with the central nervous system to ensure proper development and the maintenance of energy homeostasis. Since the formation of odor-sugar associative long-term memory (LTM) depends on the nutrient status in an animal, CCHa2 may play an essential role in linking memory and metabolic systems. Here we show that CCHa2 signals are important for consolidating appetitive memory by acting on the rewarding dopamine neurons. Genetic disruption of CCHa2 using mutant strains abolished appetitive LTM but not short-term memory (STM). A post-learning thermal suppression of CCHa2 expressing cells impaired LTM. In contrast, a post-learning thermal activation of CCHa2 cells stabilized STM induced by non-nutritious sugar into LTM. The receptor of CCHa2, CCHa2-R, was expressed in a subset of dopamine neurons that mediate reward for LTM. In accordance, the receptor expression in these dopamine neurons was required for LTM specifically. We thus concluded that CCHa2 conveys a sugar nutrient signal to the dopamine neurons for memory consolidation. Our finding establishes a direct interplay between brain reward and the putative endocrine system for long-term energy homeostasis.
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Presynaptic inhibition of dopamine neurons controls optimistic bias
Yamagata N, Ezaki T, Takahashi T, Wu H, Tanimoto H.
eLife ( eLife ) 10 2021.06 [Refereed]
Research paper (journal) Domestic Co-author
Regulation of reward signaling in the brain is critical for appropriate judgement of the environment and self. In <italic>Drosophila</italic>, the protocerebral anterior medial (PAM) cluster dopamine neurons mediate reward signals. Here, we show that localized inhibitory input to the presynaptic terminals of the PAM neurons titrates olfactory reward memory and controls memory specificity. The inhibitory regulation was mediated by metabotropic gamma-aminobutyric acid (GABA) receptors clustered in presynaptic microdomain of the PAM boutons. Cell type-specific silencing the GABA receptors enhanced memory by augmenting internal reward signals. Strikingly, the disruption of GABA signaling reduced memory specificity to the rewarded odor by changing local odor representations in the presynaptic terminals of the PAM neurons. The inhibitory microcircuit of the dopamine neurons is thus crucial for both reward values and memory specificity. Maladaptive presynaptic regulation causes optimistic cognitive bias.
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Mushroom body output differentiates memory processes and distinct memory-guided behaviors
Ichinose T, Kanno M, Wu H, Yamagata N, Sun H, Abe A, Tanimoto H.
Current Biology ( Current Biology ) 31 ( 6 ) 1294 - 1302.e4 2021.03 [Refereed]
Research paper (journal) Domestic Co-author
The mushroom body (MB) of Drosophila melanogaster has multiple functions in controlling memory and behavior.1-9 However, circuit mechanisms that generate this functional diversity are largely unclear. Here, we systematically probed the behavioral contribution of each type of MB output neuron (MBON) by blocking during acquisition, retention, or retrieval of reward or punishment memories. We evaluated the contribution using two conditioned responses: memory-guided odor choice and odor source attraction. Quantitative analysis revealed that these conditioned odor responses are controlled by different sets of MBONs. We found that the valence of memory, rather than the transition of memory steps, has a larger impact on the patterns of required MBONs. Moreover, we found that the glutamatergic MBONs forming recurrent circuits commonly contribute to appetitive memory acquisition, suggesting a pivotal role of this circuit motif for reward processing. Our results provide principles how the MB output circuit processes associative memories of different valence and controls distinct memory-guided behaviors.
◆Original paper【 display / non-display 】
Grant-in-Aid for Scientific Research 【 display / non-display 】
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Grant-in-Aid for Scientific Research(C)
Project Year： 2024.04 - 2027.03
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Fund for the Promotion of Joint International Research (Fostering Joint International Research (A))
Project Year： 2020 - 2024
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Grant-in-Aid for Young Scientists(A)
Project Year： 2017.04 - 2020.03
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Neural circuits that distinguish memory processes in the fly brain
Grant-in-Aid for Challenging Exploratory Research
Project Year： 2015.04 - 2017.03 Investigator(s): Tanimoto Hiromu, YAMAGATA Nobuhiro, ICHINOSE Toshiharu
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Direct visualization of endogenous gene expression through targeted genetic labeling
Grant-in-Aid for Scientific Research(A)
Project Year： 2014.04 - 2017.03 Investigator(s): Tanimoto Hiromu, YAMAGATA Nobuhiro
Other external funds procured 【 display / non-display 】
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Visualizing receptor activation by endo-Tango method during memory consolidation
Project Year： 2019.04 - 2020.04 Investigator(s): YAMAGATA Nobuhiro
Funding organization classification：Other
Presentations 【 display / non-display 】
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Imbalance between dopamine subsystems underlies optimism bias
Nobuhiro Yamagata, Nobuhiro Takahashi, Norihiro Katayama, Hiromu Tanimoto
The 16th Japanese Drosophila Research Conference (JDRC16) 2024.09 - 2024.09
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Activity imbalance between rewarding and punishment dopamine subsystems underlies the optimism bias
Nobuhiro Yamagata, Nobuhiro Takahashi, Norihiro Katayama, Hiromu Tanimoto
第67回 日本神経化学会大会 2024.07 - 2024.07
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Presynaptic nicotinic receptor in dopamine terminals mediates learned odor choice
Yamagata N, Ichikawa R, Takahashi N, Abe A, Katayama N, Tanimoto H
Asia Pacific Drosophila Neurobiology Conference 3 (APDNC3) 2024.02 - 2024.02
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Nicotinic acetylcholine receptor in dopamine neurons regulates learned odor choice
Yamagata N, Ichikawa R, Takahashi N, Abe A, Katayama N, Tanimoto H
日本比較生理生化学会 第45回大阪大会 2023.12 - 2023.12
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Presynaptic nicotinic receptor in dopamine neurons mediates learned odor choice
Yamagata N, Ichikawa R, Takahashi N, Abe A, Katayama N, Tanimoto H
第46回日本神経科学大会 2023.08 - 2023.08