桐明 絢 (キリアケ アヤ)

KIRIAKE Aya

写真a

所属

大学院理工学研究科  生命科学専攻  生命科学コース 

研究室住所

秋田市手形学園町1-1 理工学部6号館

研究室電話

018-889-2477

メールアドレス

メールアドレス

研究分野・キーワード

水圏生物生理活性物質 水圏生物タンパク質 

出身大学 【 表示 / 非表示

  • 2004年04月
    -
    2008年03月

    北里大学   水産学部   水産生物科学科   卒業

出身大学院 【 表示 / 非表示

  • 2010年04月
    -
    2013年03月

    東京海洋大学  海洋生命科学技術研究科  応用生命科学専攻  博士後期課程  修了

  • 2008年04月
    -
    2010年04月

    東京海洋大学  海洋科学技術研究科  食機能保全科学専攻  博士前期課程  修了

取得学位 【 表示 / 非表示

  • 東京海洋大学 -  博士(海洋科学)

  • 東京海洋大学 -  修士(海洋科学)

職務経歴(学内) 【 表示 / 非表示

  • 2021年04月
    -
    継続中

    秋田大学   大学院理工学研究科   生命科学専攻   生命科学コース   助教  

 

論文 【 表示 / 非表示

  • Localization analysis of essential oils in perilla herb (Perilla frutescens var. crispa) using derivatized mass spectrometry imaging.

    Shu Taira, Aya Kiriake-Yoshinaga, Hitomi Shikano, Ryuzoh Ikeda, Shoko Kobayashi, Kazuaki Yoshinaga

    Food science & nutrition   9 ( 5 ) 2779 - 2784   2021年05月

    The localization of essential oils, including flavor components, in perilla herb (Perilla frutescens var. crispa) were visually determined using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) imaging. The surface of a perilla leaf was peeled using a cyanoacrylate adhesion compound and contained oil glands that retained their morphology and chemical properties. We imaged the three essential oils perillaldehyde, β-caryophyllene, and rosmarinic acid (RA). Perillaldehyde was derivatized using glycine to prevent evaporation and allow its detection and imaging while localized in oil glands. β-caryophyllene also localized in the oil glands and not in the epidermis region. RA was detected throughout the leaf, including the oil glands. Quantitative data for the three essential oils were obtained by gas chromatography- or liquid chromatography-MS. The concentrations of perillaldehyde, β-caryophyllene, and RA were 12.6 ± 0.62, 0.27 ± 0.02, and 0.16 ± 0.02 [mg/g] in the paste sample of perilla herb. Peeling using a cyanoacrylate adhesion compound, and derivatization of a target such as an aroma component have great potential for mass spectrometry imaging for multiple essential oils.

    DOI PubMed

  • Simultaneous Treatment of Long-chain Monounsaturated Fatty Acid and n-3 Polyunsaturated Fatty Acid Decreases Lipid and Cholesterol Levels in HepG2 Cell.

    Kazuaki Yoshinaga, Yota Mizuno, Samanthika Senarath, Aya Yoshinaga-Kiriake, Toshiharu Nagai, Fumiaki Beppu, Seiya Tanaka, Naohiro Gotoh

    Journal of oleo science   70 ( 5 ) 731 - 736   2021年05月

    The n-3 type polyunsaturated fatty acids (n-3PUFAs), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), from fish oil exhibit health benefits such as triacylglycerol- and cholesterol-lowering effects. Some pelagic fishes contain long-chain monounsaturated fatty acids (LC-MUFAs) such as eicosenoic acid (C20:1), which exert health-promoting effects. However, no study has evaluated beneficial effects of n-3PUFA and LC-MUFA combination. Here, we investigated effects of simultaneous treatment with n-3PUFA (EPA and DHA) and LC-MUFA (cis-5-C20:1 and cis-7-C20:1) and found that n-3PUFA and LC-MUFA combination significantly decreased lipid accumulation and reduced total cholesterol in HepG2 cells. Cholesterol level was significantly lower in DHA + cis-7-C20:1 group than in DHA + EPA group. These results suggest the importance of LC-MUFA as a functional molecule in fish oil.

    DOI PubMed

  • Characterization of lactones in Wagyu (Japanese beef) and imported beef by combining solvent extraction and gas chromatography–mass spectrometry

    Kazuaki Yoshinaga, Arisa Tago, Aya Yoshinaga-Kiriake, Naohiro Gotoh

    LWT   135   2021年01月

    ISSN:0023-6438

    © 2020 Elsevier Ltd The lactone content of Wagyu beef and imported beef in Japan was estimated using a combination of solvent extraction and gas chromatography–tandem quadrupole mass spectrometry. This method revealed that the total lactone content was higher in Wagyu than in the imported beef. The most abundant lactone in the Wagyu and imported beef was δ-hexadecalactone, followed by δ-tetradecalactone, δ-dodecalactone, and δ-decalactone. The total lactone content in the Wagyu and imported beef increased approximately twofold or threefold when the extracted fats from the beef were heated. In addition, the raw beef was heated under actual cooking conditions, such as boiling at 80 °C for 2 min and roasting at 180 °C for 30 s, and found with the increased amounts of lactones. The results showed that the cooking processes increased the lactone content in the Wagyu and imported beef, and the change in the lactone content was greater in the roasted beef than in the boiled beef. These results suggest that considerable amounts of lactone precursors remain in the raw beef.

    DOI

  • Evaluation of Hepatic Uptake of OATP1B Substrates by Short Term-Cultured Plated Human Hepatocytes: Comparison With Isolated Suspended Hepatocytes

    Takashi Yoshikado, Wooin Lee, Kota Toshimoto, Kiyoe Morita, Aya Kiriake, Xiaoyan Chu, Nora Lee, Emi Kimoto, Manthena V.S. Varma, Ryota Kikuchi, Renato J. Scialis, Hong Shen, Naoki Ishiguro, Ralf Lotz, Albert P. Li, Kazuya Maeda, Hiroyuki Kusuhara, Yuichi Sugiyama

    Journal of Pharmaceutical Sciences   110 ( 1 ) 376 - 387   2021年01月

    ISSN:0022-3549

    © 2020 American Pharmacists Association® Hepatic uptake clearance has been measured in suspended human hepatocytes (SHH). Plated human hepatocytes (PHH) after short-term culturing are increasingly employed to study hepatic transport driven mainly by its higher throughput. To know pros/cons of both systems, the hepatic uptake clearances of several organic anion transporting polypeptide 1B substrates were compared between PHH and SHH by determining the initial uptake velocities or through dynamic model-based analyses. For cerivastatin, pitavastatin and rosuvastatin, initial uptake clearances (PSinf) obtained using PHH were comparable to those using SHH, while cell-to-medium concentration (C/M) ratios were 2.7- to 5.4-fold higher. For pravastatin and dehydropravastatin, hydrophilic compounds with low uptake/cellular binding, their PSinf and C/M ratio in PHH were 1.8- to 3.2-fold lower than those in SHH. These hydrophilic substrates are more prone to wash-off during the uptake study using PHH, which may explain the apparently lower uptake than SHH. The C/M ratios obtained using PHH were stable over an extended time, making PHH suitable to estimate the C/M ratios and hepatocyte-to-medium unbound concentration ratios (Kp,uu). In conclusion, PHH is useful in evaluating hepatic uptake/efflux clearances and Kp,uu of OATP1B substrates in a high-throughput manner, however, a caution is warranted for hydrophilic drugs with low uptake/cellular binding.

    DOI PubMed

  • Cell-to-Medium Concentration Ratio Overshoot in the Uptake of Statins by Human Hepatocytes in Suspension, but Not in Monolayer: Kinetic Analysis Suggesting a Partial Loss of Functional OATP1Bs

    Wooin Lee, Satoshi Koyama, Kiyoe Morita, Aya Kiriake, Ryota Kikuchi, Xiaoyan Chu, Nora Lee, Renato J. Scialis, Hong Shen, Emi Kimoto, Larry Tremaine, Naoki Ishiguro, Ralf Lotz, Kazuya Maeda, Hiroyuki Kusuhara, Yuichi Sugiyama

    AAPS Journal   22 ( 6 ) 133 - 133   2020年11月

    © 2020, The Author(s). Suspended human hepatocytes (SHH) have long been used in assessing hepatic drug uptake, while plated human hepatocytes in short-term monolayer culture (PHH) have gained use in recent years. This study aimed to cross-evaluate SHH and PHH in measuring the hepatic uptake mediated by organic anion transporting polypeptide 1Bs (OATP1Bs). We compared the time courses of cell-to-medium (C/M) concentration ratios and initial uptake clearance values of the OATP1B substrates (pitavastatin, rosuvastatin, cerivastatin, pravastatin, dehydropravastatin, and SC-62807) between SHH and PHH. For all compounds except cerivastatin, the C/M ratios in SHH displayed an apparent overshoot (an initial increase followed by a decrease) during the 180-min uptake experiment, but not in PHH. Based on the literature evidence suggesting the possible internalization of OATP1Bs in primary hepatocytes, separate experiments measured the drug uptake after varying lengths of pre-incubation in the drug-free medium. The initial uptake clearances of pitavastatin and rosuvastatin declined in SHH beyond an apparent threshold time of 20-min drug-free pre-incubation, but not in PHH. Kinetic modeling quantitatively captured the decline in the active uptake clearance in SHH, and more than half of the active uptake clearances of pitavastatin and rosuvastatin were prone to loss during the 180-min uptake experiment. These results suggested a partial, time-delayed loss of the functional OATP1Bs in SHH upon prolonged incubation. Our results indicate that PHH is more appropriate for experiments where a prolonged incubation is required, such as estimation of unbound hepatocyte-to-medium concentration ratio (Kp,uu) at the steady-state.

    DOI PubMed

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Book(書籍) 【 表示 / 非表示

  • 水圏生物タンパク質科学の新展開 = Recent progress in protein studies on aquatic organisms

    尾島, 孝男, 落合, 芳博, 日本水産学会 (担当: その他 )

    恒星社厚生閣  2020年10月 ISBN: 9784769916574

    CiNii

MISC(査読の無い研究業績) 【 表示 / 非表示

  • Ⅱ-3. 魚介類の毒素タンパク質

    長島 裕二, 桐明 絢

    日本水産学会誌 ( 公益社団法人 日本水産学会 )  83 ( 5 ) 821 - 821   2017年

    未設定

    DOI CiNii