桐明 絢 (キリアケ アヤ)

KIRIAKE Aya

写真a

所属

大学院理工学研究科  生命科学専攻  生命科学コース 

研究室住所

秋田市手形学園町1-1 理工学部6号館

研究室電話

018-889-2477

メールアドレス

メールアドレス

研究分野・キーワード

水圏生物生理活性物質 水圏生物タンパク質 

出身大学 【 表示 / 非表示

  • 2004年04月
    -
    2008年03月

    北里大学   水産学部   水産生物科学科   卒業

出身大学院 【 表示 / 非表示

  • 2010年04月
    -
    2013年03月

    東京海洋大学  海洋生命科学技術研究科  応用生命科学専攻  博士後期課程  修了

  • 2008年04月
    -
    2010年04月

    東京海洋大学  海洋科学技術研究科  食機能保全科学専攻  博士前期課程  修了

取得学位 【 表示 / 非表示

  • 東京海洋大学 -  博士(海洋科学)

  • 東京海洋大学 -  修士(海洋科学)

職務経歴(学内) 【 表示 / 非表示

  • 2021年04月
    -
    継続中

    秋田大学   大学院理工学研究科   生命科学専攻   生命科学コース   助教  

 

論文 【 表示 / 非表示

  • Visualization of dietary docosahexaenoic acid in whole-body zebrafish using matrix-assisted laser desorption/ionization mass spectrometry imaging.

    Kazuaki Yoshinaga, Yuka Usami, Aya Yoshinaga-Kiriake, Hitomi Shikano, Shu Taira, Reiko Nagasaka, Seiya Tanaka, Naohiro Gotoh

    The Journal of nutritional biochemistry   100   108897 - 108897   2022年02月  [査読有り]

    国内共著

    Zebrafish models have been developed for several studies involving lipid metabolism and lipid-related diseases. In the present study, the migration of dietary docosahexaenoic acid (DHA) in whole-body zebrafish was estimated by stable-isotope tracer and matrix-assisted laser desorption/ionization mass spectrometry imaging. Administration of 1-13C-2,2-D2-labeled DHA ((+3)DHA) ethyl ester to male zebrafish was conducted to evaluate its accumulation, migration, and distribution in the body. The (+3)DHA content in the body of zebrafish after administering (+3)DHA for 10 and 15 d was significantly higher than that in the control group. (+3)DHA was observed as a constituent of phosphatidylcholine (PC) in the intestine of zebrafish that were administered (+3)DHA for 5 and 10 d. (+3)DHA-containing PC tended to accumulate in the intestines of zebrafish administered (+3)DHA for 1 d, indicating that recombination of (+3)DHA from ethyl ester to PC occurs quickly at intestine. After administration for 15 d, (+3)DHA-containing PC accumulated in the intestine, liver, and muscle of whole-body zebrafish. In contrast, (+3)DHA-containing PC was not detected in the brain. These results showed that dietary DHA is initially constructed into PC as a structural component of intestinal cell membranes and gradually migrates into peripheral tissues such as muscle.

    DOI PubMed

  • Localization analysis of essential oils in perilla herb (Perilla frutescens var. crispa) using derivatized mass spectrometry imaging.

    Shu Taira, Aya Kiriake-Yoshinaga, Hitomi Shikano, Ryuzoh Ikeda, Shoko Kobayashi, Kazuaki Yoshinaga

    Food science & nutrition   9 ( 5 ) 2779 - 2784   2021年05月  [査読有り]

    国内共著

    The localization of essential oils, including flavor components, in perilla herb (Perilla frutescens var. crispa) were visually determined using matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) imaging. The surface of a perilla leaf was peeled using a cyanoacrylate adhesion compound and contained oil glands that retained their morphology and chemical properties. We imaged the three essential oils perillaldehyde, β-caryophyllene, and rosmarinic acid (RA). Perillaldehyde was derivatized using glycine to prevent evaporation and allow its detection and imaging while localized in oil glands. β-caryophyllene also localized in the oil glands and not in the epidermis region. RA was detected throughout the leaf, including the oil glands. Quantitative data for the three essential oils were obtained by gas chromatography- or liquid chromatography-MS. The concentrations of perillaldehyde, β-caryophyllene, and RA were 12.6 ± 0.62, 0.27 ± 0.02, and 0.16 ± 0.02 [mg/g] in the paste sample of perilla herb. Peeling using a cyanoacrylate adhesion compound, and derivatization of a target such as an aroma component have great potential for mass spectrometry imaging for multiple essential oils.

    DOI PubMed

  • Simultaneous Treatment of Long-chain Monounsaturated Fatty Acid and n-3 Polyunsaturated Fatty Acid Decreases Lipid and Cholesterol Levels in HepG2 Cell.

    Kazuaki Yoshinaga, Yota Mizuno, Samanthika Senarath, Aya Yoshinaga-Kiriake, Toshiharu Nagai, Fumiaki Beppu, Seiya Tanaka, Naohiro Gotoh

    Journal of oleo science   70 ( 5 ) 731 - 736   2021年05月  [査読有り]

    国内共著

    The n-3 type polyunsaturated fatty acids (n-3PUFAs), including eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), from fish oil exhibit health benefits such as triacylglycerol- and cholesterol-lowering effects. Some pelagic fishes contain long-chain monounsaturated fatty acids (LC-MUFAs) such as eicosenoic acid (C20:1), which exert health-promoting effects. However, no study has evaluated beneficial effects of n-3PUFA and LC-MUFA combination. Here, we investigated effects of simultaneous treatment with n-3PUFA (EPA and DHA) and LC-MUFA (cis-5-C20:1 and cis-7-C20:1) and found that n-3PUFA and LC-MUFA combination significantly decreased lipid accumulation and reduced total cholesterol in HepG2 cells. Cholesterol level was significantly lower in DHA + cis-7-C20:1 group than in DHA + EPA group. These results suggest the importance of LC-MUFA as a functional molecule in fish oil.

    DOI PubMed

  • Evaluation of Hepatic Uptake of OATP1B Substrates by Short Term-Cultured Plated Human Hepatocytes: Comparison With Isolated Suspended Hepatocytes

    Takashi Yoshikado, Wooin Lee, Kota Toshimoto, Kiyoe Morita, Aya Kiriake, Xiaoyan Chu, Nora Lee, Emi Kimoto, Manthena V.S. Varma, Ryota Kikuchi, Renato J. Scialis, Hong Shen, Naoki Ishiguro, Ralf Lotz, Albert P. Li, Kazuya Maeda, Hiroyuki Kusuhara, Yuichi Sugiyama

    Journal of Pharmaceutical Sciences   110 ( 1 ) 376 - 387   2021年01月  [査読有り]

    ISSN:0022-3549

    国際共著

    © 2020 American Pharmacists Association® Hepatic uptake clearance has been measured in suspended human hepatocytes (SHH). Plated human hepatocytes (PHH) after short-term culturing are increasingly employed to study hepatic transport driven mainly by its higher throughput. To know pros/cons of both systems, the hepatic uptake clearances of several organic anion transporting polypeptide 1B substrates were compared between PHH and SHH by determining the initial uptake velocities or through dynamic model-based analyses. For cerivastatin, pitavastatin and rosuvastatin, initial uptake clearances (PSinf) obtained using PHH were comparable to those using SHH, while cell-to-medium concentration (C/M) ratios were 2.7- to 5.4-fold higher. For pravastatin and dehydropravastatin, hydrophilic compounds with low uptake/cellular binding, their PSinf and C/M ratio in PHH were 1.8- to 3.2-fold lower than those in SHH. These hydrophilic substrates are more prone to wash-off during the uptake study using PHH, which may explain the apparently lower uptake than SHH. The C/M ratios obtained using PHH were stable over an extended time, making PHH suitable to estimate the C/M ratios and hepatocyte-to-medium unbound concentration ratios (Kp,uu). In conclusion, PHH is useful in evaluating hepatic uptake/efflux clearances and Kp,uu of OATP1B substrates in a high-throughput manner, however, a caution is warranted for hydrophilic drugs with low uptake/cellular binding.

    DOI PubMed

  • Characterization of lactones in Wagyu (Japanese beef) and imported beef by combining solvent extraction and gas chromatography–mass spectrometry

    Kazuaki Yoshinaga, Arisa Tago, Aya Yoshinaga-Kiriake, Naohiro Gotoh

    LWT   135   2021年01月  [査読有り]

    ISSN:0023-6438

    国内共著

    © 2020 Elsevier Ltd The lactone content of Wagyu beef and imported beef in Japan was estimated using a combination of solvent extraction and gas chromatography–tandem quadrupole mass spectrometry. This method revealed that the total lactone content was higher in Wagyu than in the imported beef. The most abundant lactone in the Wagyu and imported beef was δ-hexadecalactone, followed by δ-tetradecalactone, δ-dodecalactone, and δ-decalactone. The total lactone content in the Wagyu and imported beef increased approximately twofold or threefold when the extracted fats from the beef were heated. In addition, the raw beef was heated under actual cooking conditions, such as boiling at 80 °C for 2 min and roasting at 180 °C for 30 s, and found with the increased amounts of lactones. The results showed that the cooking processes increased the lactone content in the Wagyu and imported beef, and the change in the lactone content was greater in the roasted beef than in the boiled beef. These results suggest that considerable amounts of lactone precursors remain in the raw beef.

    DOI

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Book(書籍) 【 表示 / 非表示

  • 水圏生物タンパク質科学の新展開 = Recent progress in protein studies on aquatic organisms

    尾島, 孝男, 落合, 芳博, 日本水産学会 (担当: 分担執筆 )

    恒星社厚生閣  2020年10月 ISBN: 9784769916574

    学術書

    CiNii

MISC(査読の無い研究業績) 【 表示 / 非表示

  • Ⅱ-3. 魚介類の毒素タンパク質

    長島 裕二, 桐明 絢

    日本水産学会誌 ( 公益社団法人 日本水産学会 )  83 ( 5 ) 821 - 821   2017年  [査読有り]  [依頼有り]

    講演資料等(セミナー,チュートリアル,講習,講義他)   国内共著

    DOI

  • 魚介類の毒とその特徴(特集 魚介類の毒とその管理 : 安全確保の肝所)

    長島 裕二、桐明 絢

    月刊アクアネット2016年12月号 ( 有限会社湊文社出版 )    22 - 26   2016年12月

    総説・解説(商業誌)   国内共著

  • しらすへのフグ稚魚混入

    長島 裕二、桐明 絢

    全水卸平成28年7月号 ( 一般社団法人全国水産卸協会 )  356   8 - 11   2016年07月  [依頼有り]

    総説・解説(その他)   国内共著

  • 海洋危険生物 食べて中毒;魚の毒

    長島裕二、桐明 絢

    中毒研究   29 ( 1 ) 3 - 9   2016年03月  [査読有り]  [依頼有り]

    総説・解説(学術雑誌)   国内共著

学術関係受賞 【 表示 / 非表示

  • 水産学奨励賞

    2022年01月   公益財団法人 日本水産学会   魚類刺毒に関する生化学的研究

    受賞者:  桐明 絢

科研費(文科省・学振)獲得実績 【 表示 / 非表示

  • DHAおよびEPA過酸化物に対する安定同位体を用いた体内動態の解明

    若手研究

    研究期間:  2021年04月  -  継続中  代表者:  桐明 絢

  • ラベル化同位体を用いた多価不飽和脂肪酸過酸化物の体内動態視覚化技術の開発

    若手研究

    研究期間:  2019年04月  -  2021年03月  代表者:  桐明 絢

  • アレルギー性疾患に関与する食品中のトランス脂肪酸位置異性体の解明

    研究活動スタート支援

    研究期間:  2018年04月  -  2019年03月  代表者:  桐明 絢

その他競争的資金獲得実績 【 表示 / 非表示

  • 刺毒魚のもつ毒成分の分布と構造の解明

    提供機関:  一般財団法人守谷育英会  一般財団法人守谷育英会研究助成

    研究期間: 2014年04月  -  2016年03月  代表者:  桐明 絢

    資金支給機関区分:その他

  • ミトコンドリアDNAを用いたテトラミン食中毒原因巻貝の種判別法の開発

    提供機関:  公益財団法人 日本科学協会  平成26年度笹川科学研究助成

    研究期間: 2014年04月  -  2015年03月  代表者:  桐明 絢

    資金支給機関区分:その他

学会等発表 【 表示 / 非表示

  • オニグルミとペルシャグルミの脂肪酸組成および酸化安定性の比較

    桐明 絢、高田 大輔、則藤 孝志、牧 雅康、望月 翔太、吉永 和明

    日本農芸化学会2022年度大会  2022年03月  -  2022年03月   

  • トランス脂肪酸位置異性体のRAW264.7細胞に対する炎症性の検討

    桐明絢、吉永和明、後藤直宏

    日本油化学会第60回年会  2021年09月  -  2021年09月   

  • 魚類のもつ刺毒の正体は何か?~タンパク毒の性状と構造解析~

    桐明 絢  [招待有り]

    生物工学会北日本支部 2021年度第一回オンライン若手シンポジウム  2021年08月  -  2021年08月   

  • 魚類のもつ刺毒の正体は何か?~タンパク毒の性状と構造~

    桐明 絢  [招待有り]

    北東北国立3大学「農工連携」をテーマとした研究者交流会  2021年05月  -  2021年05月   

 

学外の社会活動(高大・地域連携等) 【 表示 / 非表示

  • 秋田北高等学校数理探求クラス1年生対象 秋田大学生命科学科実験・模擬講義研修

    2021年11月