YOKOTA Saki

写真a

Affiliation

Graduate School of Engineering Science  Department of Materials Science  Applied Chemistry Course 

Research Fields, Keywords

biotechnology

Graduating School 【 display / non-display

  • 2006.04
    -
    2010.03

    Yamagata University   Faculty of Science   Graduated

Graduate School 【 display / non-display

  •  
    -
    2019.09

    Akita University  Graduate School of Engineering and Resource Science  Doctor's Degree Program  Completed

  • 2010.04
    -
    2012.03

    Yamagata University  Graduate School, Division of Science  Master's Course  Completed

Campus Career 【 display / non-display

  • 2012.04
    -
    Now

    Akita University   Graduate School of Engineering and Resource Science   Department of Materials-process Engineering and Applied Chemistry Environ   Assistant Professor  

Research Field (grants-in-aid-for-scientific-research classification) 【 display / non-display

  • Biofunction/Bioprocess

 

Published Papers 【 display / non-display

  • Preparation of highly selective sorbents composed of peptides and silica using novel molecular imprinting technology for target metal ions

    Stevens M.G., Yokota Saki, Gotoh Takeshi.

    Journal of Chemical Engineering of Japan ( Journal of Chemical Engineering of Japan )  53 ( 9 ) 485 - 493   2020.09  [Refereed]

    Domestic Co-author

    <p>A novel molecular imprinting technology was developed to prepare highly selective sorbents, composed of adhesive peptides and microporous silica particles, for the adsorption of target metal ions from aqueous environments. Using solid-phase peptide-synthesis, tyrosine-, lysine-, and histidine-containing peptides were tailored to contain tyrosine-, lysine- and histidine-rich regions. The tyrosine residues of the peptide were converted to 3,4-dihydroxy-L-phenylalanine (DOPA) residues to give the peptides adhesive properties mimicking the adhesion of mussel-foot proteins to wet surfaces. The resulting DOPA-peptides were incubated with target metal ions Ni(II), Cu(II), Co(II), or Zn(II), and subsequently with microporous silica particles, at pH 8.0 and 25°C. Almost all the DOPA-peptides, with the metal ions, strongly attached to the particles within 24 h and were not detached from them upon washing with an aqueous solution of pH 1–12. However, the metal ions were easily released from the particles at a pH below 7.0, implying that imprinted cavities from the target metal ions, created on the peptide-immobilized silica particles, remained after the removal of the template metal ions. The metal adsorption characteristics of the resulting particles, which were termed metal-imprinted peptide–silica particles (metal-iPSPs), were investigated. The target metal ions were adsorbed to metal-iPSPs at a pH of 8.0. The adsorption kinetics fit a pseudo-second-order model well. The adsorption isotherm was well approximated using the Langmuir model, indicating that the metal-iPSP surface was homogeneous, adsorption sites were equivalent, and the coverage formed a monolayer. The metal-iPSPs exhibited a significant and high selectivity for the imprinted target metal ions in the presence of an equimolar quantity of competitive metal ions.</p>

    DOI CiNii

  • B38-CAP is a bacteria-derived ACE2-like enzyme that suppresses hypertension and cardiac dysfunction

    Takafumi Minato, Satoru Nirasawa, Teruki Sato, Tomokazu Yamaguchi, Midori Hoshizaki, Tadakatsu Inagaki, Kazuhiko Nakahara, Tadashi Yoshihashi, Ryo Ozawa , Saki Yokota, Miyuki Natsui, Souichi Koyota, Taku Yoshiya, Kumiko Yoshizawa-Kumagaye, Satoru Motoyama, Takeshi Gotoh, Yoshikazu Nakaoka, Josef M Penninger, Hiroyuki Watanabe, Yumiko Imai, Saori Takahashi, Keiji Kuba

    Nature Communications ( Nature Communications )  11 ( 1 )   2020.02  [Refereed]

    International Co-author

    DOI

  • Effects of rubber elongation factor and small rubber particle protein from rubber-producing plants on lipid metabolism in Saccharomyces cerevisiae

    Yokota Saki., Gotoh Takeshi.

    Journal of Bioscience and Bioengineering ( Journal of Bioscience and Bioengineering )  128 ( 5 ) 585 - 592   2019.11  [Refereed]

    Domestic Co-author

    DOI

  • Aggregation properties as potential markers for classifying rubber particle proteins

    Yokota Saki, Gotoh Takeshi

    Atlas of Science     2019.01  [Invited]

    Domestic Co-author

  • Cloning and Aggregation Characterization of Rubber Elongation Factor and Small Rubber Particle Protein from Ficus carica

    Yokota Saki, Suzuki, Yurina, Saitoh Keisuke, Kitajima Sakihito, Ohya Norimasa, Gotoh, Takeshi

    Molecular Biotechnology   60 ( 2 ) 83 - 91   2018.02  [Refereed]

    Domestic Co-author

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Grant-in-Aid for Scientific Research 【 display / non-display

  • Grant-in-Aid for Early-Career Scientists

    Project Year: 2019.04  -  2021.03 

Presentations 【 display / non-display

  • Preparation of selective sorbents for selenium(IV) and selenium(VI) composed of lysine-peptide and porous silica using molecular imprinting technology

    Stevens Morlu GF,横田早希,山崎正貴,後藤猛

    化学工学会第51回秋季大会  2020.09  -  2020.09 

  • Expression of rubber particle proteins in Saccharomyces cerevisiae and effect on lipid droplet formation

    2018.03  -  2018.03 

  • Cloning and Comparison of Aggregation Properties of HbREF Like Proteins from Ficus carica

    S. YOKOTA, Y. SUZUKI, K. SAITOH, S. KITAJIMA, N. OHYA, T. GOTOH

    第8回素材物性学国際会議  2017.10  -  2017.10 

  • Exploration of a Proximal Protein to Baculovirus GP64 in Sf9 insect cells

    Yokota, S. and Gotoh, T.

    17th International Biotechnology Symposium and Exhibition  2016.10  -  2016.10 

  • Oxygen transfer performance in medium atomization bioreactor using a spinning inverted corn

    Gotoh, T., Nakanishi, S., Sato, Y., Yokota. S., Adachi, T.

    17th International Biotechnology Symposium and Exhibition  2016.10  -  2016.10 

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