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Affiliation |
Hospital SurgeryⅡ |
Graduating School 【 display / non-display 】
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-2009.03
Akita University Faculty of Medicine Graduated
Campus Career 【 display / non-display 】
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2019.04-Now
Akita University Hospital SurgeryⅡ Lecturer
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2017.12-2019.03
Akita University Hospital SurgeryⅡ Assistant Professor
Thesis for a degree 【 display / non-display 】
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Novel rapid-immunohistochemistry using an alternating current electric field for intraoperative diagnosis of sentinel lymph nodes in breast cancer
Kaori Terata, Hajime Saito, Hiroshi Nanjo, Yuko Hiroshima, Satoru Ito, Kasumi Narita, Yoichi Akagami, Ryuta Nakamura, Hayato Konno, Aki Ito, Satoru Motoyama & Yoshihiro Minamiya
2017.09
Domestic Co-author
Research Achievements 【 display / non-display 】
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Intraoperative diagnosis with novel rapid-immunohistochemistry (R-IHC)
NANJO Hiroshi, AKAGAMI Yoichi, NAKAMURA Rhuta, HIROSHIMA Yuko, TERADA Kaori, IMAI Kazuhiro, Minamiya Yoshihiro
The Proceedings of Mechanical Engineering Congress, Japan ( The Japan Society of Mechanical Engineers ) 2019 ( 0 ) 2019
<p>Immunohistochemistry (IHC) is the most common application of immunostaining. It is widely used in the disgnosis of abnormal cells such as those found in cancerous tumors. Intraoperative pathological diagnosis is also important in selecting suitable surgery for cancer patients. We performed intraoperative diagnosis of 830 cases in Akita University Hospital., with a novel method of rapid-immunohistochemistory (R-IHC) using an alternating current electric field. The results were very good, R-IHC detected an objective evidence that were undetected using conventional HE staining. So we can diagnose degree of malignancy, histological type of cancer, and lymph node metastasis more accurately.</p>
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Intraoperative diagnosis with novel rapid-immunohistochemistry (R-IHC)
NANJO Hiroshi, AKAGAMI Yoichi, NAKAMURA Rhuta, HIROSHIMA Yuko, TERADA Kaori, IMAI Kazuhiro, Minamiya Yoshihiro
The Proceedings of Mechanical Engineering Congress, Japan ( The Japan Society of Mechanical Engineers ) 2019 ( 0 ) W02101 2019
<p>Immunohistochemistry (IHC) is the most common application of immunostaining. It is widely used in the disgnosis of abnormal cells such as those found in cancerous tumors. Intraoperative pathological diagnosis is also important in selecting suitable surgery for cancer patients. We performed intraoperative diagnosis of 830 cases in Akita University Hospital., with a novel method of rapid-immunohistochemistory (R-IHC) using an alternating current electric field. The results were very good, R-IHC detected an objective evidence that were undetected using conventional HE staining. So we can diagnose degree of malignancy, histological type of cancer, and lymph node metastasis more accurately.</p>
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HOSHINO Iku, SATO Yusuke, MOTOYAMA Satoru, NANJO Hiroshi, AKAGAMI Yoichi, MINAMIYA Yoshihiro, IMAI Kazuhiro, NAKAMURA Ryuta, SAITO Yoshitaro, FUJISHIMA Satoshi, KURIHARA Nobuyasu, Wakamatsu Yuki, SAITO Hajime, TERATA Kaori
Journal of the Japan Society for Precision Engineering ( The Japan Society for Precision Engineering ) 84 ( 4 ) 383 - 387 2018
<p>It is widely recognized that pathology is the most important factor for staging and selecting effective chemotherapy for patients with cancer. Immunohistochemistry (IHC) is a reliable screening method, but intra-operative diagnosis by frozen section with IHC is not possible because IHC takes approximately 6 hours. We recently developed a rapid-IHC method that makes use of an alternating current (AC) electric field to facilitate the antigen-antibody reaction, and reported its usefulness for detection of lung cancer and breast cancer. The antibody is mixed within microdroplets as the voltage is switched on and off at specific intervals. The resultant Coulomb force stirs the diluted solution on the sections, which increases the opportunity for contact. The rapid-IHC device reduces the time required for IHC as well as the amount of antibody required for analyses. The device may also be applied for in situ hybridization (ISH); however, ISH requires a warm temperature, such as 37℃, and a longer processing time than the rapid-IHC method. Consequently, evaporation can be a problem during ISH. The aim of the present study was to evaluate the utility and reliability of an oil cover and cover cap made of polyethylene terephthalate (PET) for preventing evaporation due to the AC electric field applied to facilitate the antigen-antibody reaction. We have shown that both the oil cover and PET cover cap prevented evaporation when using the rapid-IHC device.</p>